Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164230_pkiw CCATATTTTATGAAGGGATCTCAATGGTACCG(+AGGACA)GATCCCTGTGGCTATAGCCGGTAGTACCTTA NGG 10 8 17 8 24 0 100 5 10
    240613164229_1py7 GGAGCCACAAGGAGTGGGCTTCATGCATTATG(+AGGACA)GATCCCGACCCCCGCCACTGTGGGCTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240613164227_3hmz TCCTGGAGCAAGCCCACAGTGGCGGGGGTCGG(+AGGACA)GATCCATAATGCATGAAGCCCACTCCTTGTG NGG 10 8 17 8 24 0 100 5 10
    240613164225_2ags CCAGCAAGAAGGGGGAGCAGGGGACGAGGGGG(+AGGACA)GTACCTATCCTTCCTGAACGGACAATGGGGG NGG 10 8 17 8 24 0 100 5 10
    240613164223_ruo9 CATCACCCCCATTGTCCGTTCAGGAAGGATAG(+AGGACA)GTACCCCCCTCGTCCCCTGCTCCCCCTTCTT NGG 10 8 17 8 24 0 100 5 10
    240613164222_3rnz AAAGTATTTCATTATGCTGGCATGCCCAGTAG(+AGGACA)GATCCCCTGAGGCCCAGATGGGCCACATGTA NGG 10 8 17 8 24 0 100 5 10
    240613164220_c7d1 TGGTATACATGTGGCCCATCTGGGCCTCAGGG(+AGGACA)GATCCTACTGGGCATGCCAGCATAATGAAAT NGG 10 8 17 8 24 0 100 5 10
    240613164219_mafg CCACCTCACACCCAATATGTCCAAAGTCTCAG(+AGGACA)GATCCAGGAATAAACAAAGCCGGGAGAATCT NGG 10 8 17 8 24 0 100 5 10
    240613164216_8n1c CACGCAGATTCTCCCGGCTTTGTTTATTCCTG(+AGGACA)GATCCTGAGACTTTGGACATATTGGGTGTGA NGG 10 8 17 8 24 0 100 5 10
    240613164214_6m2v GGCGATGGCGCAGATGGTGCGTTCAAACCCGG(+AGGACA)GATCCCAGCTGTCCACTACACCAATGGGACA NGG 10 8 17 8 24 0 100 5 10