Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164302_2eta CTGCTGAGCCTGACAACCTGATGCTATTCCTG(+AGGACA)GTACCACATGCTGGAAAGCGAAAACTCTCCC NGG 10 8 17 8 24 0 100 5 10
    240613164301_va44 ACAAATAACTCATATGCAAAGCCAATTCGCAG(+AGGACA)GATCCTGGAAACGTATCAGGATACTATCTCA NGG 10 8 17 8 24 0 100 5 10
    240613164259_tfha GCTTTTGAGATAGTATCCTGATACGTTTCCAG(+AGGACA)GATCCTGCGAATTGGCTTTGCATATGAGTTA NGG 10 8 17 8 24 0 100 5 10
    240613164258_928g GCCACATGGTGGCTCACAACCATCTGTAATGG(+AGGACA)GATCCGATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613164256_qhan GATGTCTTCAGACACACCAGAAGAGGGCATCG(+AGGACA)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240613164255_sgt9 CTCAGGGAGAGACTTGCAAAACAAACTGTGTG(+AGGACA)GATCCTGCAGACCAACATTAAAGGTTGACCT NGG 10 8 17 8 24 0 100 5 10
    240613164254_3vei GCCAAAGGTCAACCTTTAATGTTGGTCTGCAG(+AGGACA)GATCCACACAGTTTGTTTTGCAAGTCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240613164252_8jco GGCCCTCAGGCCCACACTCCCTAGGTGGCCTG(+AGGACA)GATCCTGCAGACCTTGCCCATTCTCCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240613164251_h6jk AGGAGACACTGGAGAATGGGCAAGGTCTGCAG(+AGGACA)GATCCAGGCCACCTAGGGAGTGTGGGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240613164250_zr4z GAGTTCATCTGCCCTTCATGTTTTATGCGAAG(+AGGACA)GTACCTCCTTTGTACTTAGAAAAAAAAAGAC NGG 10 8 17 8 24 0 100 5 10