Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164348_a3qq TTTCTACTTCCTTATGCATCCACTCCAGCTTG(+AGGACA)GTACCCTCTTAAAGTCCAACCAACACAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613164347_vzhe ACAGAGTGTACAACTCAGGGTAGCAACTGAAG(+AGGACA)GTACCTGGTCAAAATGACACCTACAGATGAG NGG 10 8 17 8 24 0 100 5 10
    240613164346_dz5m AAAATCTCATCTGTAGGTGTCATTTTGACCAG(+AGGACA)GTACCTTCAGTTGCTACCCTGAGTTGTACAC NGG 10 8 17 8 24 0 100 5 10
    240613164344_454d TATAGTAAAGTCAATGATGCTACATTCACTAG(+AGGACA)GATCCAGATACTGATTGTCTGTGAAGGACTA NGG 10 8 17 8 24 0 100 5 10
    240613164343_yz94 CTCGTTAGTCCTTCACAGACAATCAGTATCTG(+AGGACA)GATCCTAGTGAATGTAGCATCATTGACTTTA NGG 10 8 17 8 24 0 100 5 10
    240613164342_jnw7 TTGAGTCAAACCATGGTACTTGAATCATTGAG(+AGGACA)GATCCAAGTCATGATTTGAATTCTGTTGCCA NGG 10 8 17 8 24 0 100 5 10
    240613164340_9nmr AAAAATGGCAACAGAATTCAAATCATGACTTG(+AGGACA)GATCCTCAATGATTCAAGTACCATGGTTTGA NGG 10 8 17 8 24 0 100 5 10
    240613164338_7k5e GCTTTCAATCATCTGTAACTCCAGTTCCAATG(+AGGACA)GATCCAACACCTTATAGCCTCTGGTACACAT NGG 10 8 17 8 24 0 100 5 10
    240613164337_dhlp TATGCATGTGTACCAGAGGCTATAAGGTGTTG(+AGGACA)GATCCATTGGAACTGGAGTTACAGATGATTG NGG 10 8 17 8 24 0 100 5 10
    240613164336_bv4h CATACAGAGGGATACCACTGCTGTTGGGTCTG(+AGGACA)GTACCAGACACTCAGGCCCTTCATCTTCTTC NGG 10 8 17 8 24 0 100 5 10