Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164451_sz7z CTTCAGGTGATAAAACCCAACCTAAGAAGTTG(+AGGACA)GTACCAGGAGGGGAGTATTGGTGTGAAAGGC NGG 10 8 17 8 24 0 100 5 10
    240613164449_cx2k GGTACTGTTAAGACCCACACCTGTGGCTCTCG(+AGGACA)GTACCTTTTGGGGAGGTGAGGCAGAGCCGAA NGG 10 8 17 8 24 0 100 5 10
    240613164448_aocx TATTTTTCGGCTCTGCCTCACCTCCCCAAAAG(+AGGACA)GTACCGAGAGCCACAGGTGTGGGTCTTAACA NGG 10 8 17 8 24 0 100 5 10
    240613164447_2evi TCAGCAGTTTAGGGAGGACTGTGCAAAGGAAG(+AGGACA)GTACCTTTTTGAACCACAACAGGTGTTTGCC NGG 10 8 17 8 24 0 100 5 10
    240613164446_uosj ACACAGGCAAACACCTGTTGTGGTTCAAAAAG(+AGGACA)GTACCTTCCTTTGCACAGTCCTCCCTAAACT NGG 10 8 17 8 24 0 100 5 10
    240613164444_x1xi TGTGACCAATTATATGGTCAGTTTTGGAGAAG(+AGGACA)GTACCATAAGGTGCTGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10
    240613164443_wvwv AAAAGGATATACCTTCTTCTCAGCACCTTATG(+AGGACA)GTACCTTCTCCAAAACTGACCATATAATTGG NGG 10 8 17 8 24 0 100 5 10
    240613164441_ef57 TTCCTCTCAAGTGCTGGGATTAATGCAATGGG(+AGGACA)GTACCAGGACTGGCAATAAAATTACCTACTT NGG 10 8 17 8 24 0 100 5 10
    240613164439_x9ta CTTAGAAGTAGGTAATTTTATTGCCAGTCCTG(+AGGACA)GTACCCCATTGCATTAATCCCAGCACTTGAG NGG 10 8 17 8 24 0 100 5 10
    240613164438_g2yi TGGGAAGACCAGCCCCCACCCAGAGAATATTG(+AGGACA)GATCCTTGGATAAAAATCACAGACACAATGC NGG 10 8 17 8 24 0 100 5 10