Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164418_c4wf TCCTCAGAAAATTGGACATAGTACTACCGGAG(+AGGACA)GATCCCGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240613164416_b19v GTTAAAGGACTCCTGAGAAGTTACAGACTCAG(+AGGACA)GATCCTTATAATTATTTGGGTTTTAGACTTA NGG 10 8 17 8 24 0 100 5 10
    240613164415_ow2i ATCTGTAAGTCTAAAACCCAAATAATTATAAG(+AGGACA)GATCCTGAGTCTGTAACTTCTCAGGAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240613164414_l5nw ATCCCTTCTACTAAGTGCATACATGTAGTTTG(+AGGACA)GATCCATATTTCAATTCACTGTCAAGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613164412_suop GTCATAGAAACTTGACAGTGAATTGAAATATG(+AGGACA)GATCCAAACTACATGTATGCACTTAGTAGAA NGG 10 8 17 8 24 0 100 5 10
    240613164411_wh1p ACAAGACCAAAGGACAAAGGAAAAAAATCTTG(+AGGACA)GATCCACAAAATTCTGAAAATTATTGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240613164409_f7vp AATACCTCCTCAATAATTTTCAGAATTTTGTG(+AGGACA)GATCCAAGATTTTTTTCCTTTGTCCTTTGGT NGG 10 8 17 8 24 0 100 5 10
    240613164408_p1na TGTGTGTGAAACCAGAGATGAAAGCATGATTG(+AGGACA)GATCCTTTATACTTGGTAAACAAACCTCATG NGG 10 8 17 8 24 0 100 5 10
    240613164407_98tg ATAGACATGAGGTTTGTTTACCAAGTATAAAG(+AGGACA)GATCCAATCATGCTTTCATCTCTGGTTTCAC NGG 10 8 17 8 24 0 100 5 10
    240613164405_mmm0 ATCCCTTCTACTAAGTGCATACATGTAGTTTG(+AGGACA)GATCCATATTTCAATTCACTGTCAAGTTTCT NGG 10 8 17 8 24 0 100 5 10