Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164519_vf6k TCTTCGACTAGAACTGGTGAGATATCTTTGGG(+AGGACA)GTACCCAGCTCGTTTGCTGTACCCAGTTGTG NGG 10 8 17 8 24 0 100 5 10
    240613164518_83rl TTAAAAGGCACTTATAATAAAGAGAGCACATG(+AGGACA)GATCCATCCACTAGACTAAGGCGGGAATGGA NGG 10 8 17 8 24 0 100 5 10
    240613164516_qkuo AATTTTCCATTCCCGCCTTAGTCTAGTGGATG(+AGGACA)GATCCATGTGCTCTCTTTATTATAAGTGCCT NGG 10 8 17 8 24 0 100 5 10
    240613164515_zw6q ATCTTTAAAAAATATTGATGGTATATTGCTTG(+AGGACA)GTACCAAAAGAAAAGAAAAAATGAAATTCAC NGG 10 8 17 8 24 0 100 5 10
    240613164514_04k8 CCTGTGTGAATTTCATTTTTTCTTTTCTTTTG(+AGGACA)GTACCAAGCAATATACCATCAATATTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240613164513_j3i8 TTTGGTTATTCAGATCTTATCCAACACAATTG(+AGGACA)GATCCATACTAAAAAGAAACCAAACCTTTGT NGG 10 8 17 8 24 0 100 5 10
    240613164511_5qxw TCTTCACAAAGGTTTGGTTTCTTTTTAGTATG(+AGGACA)GATCCAATTGTGTTGGATAAGATCTGAATAA NGG 10 8 17 8 24 0 100 5 10
    240613164510_2duq TGGGAGGAACCAACCCATCTCTGTTGAGCAAG(+AGGACA)GATCCTGGATTGTATAAAAGGAGAAAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240613164508_4mjz CTCAGTTCCCTTTCTCCTTTTATACAATCCAG(+AGGACA)GATCCTTGCTCAACAGAGATGGGTTGGTTCC NGG 10 8 17 8 24 0 100 5 10
    240613164507_1szm ATGCAGTTCTTACATAAATAACTATTGATAGG(+AGGACA)GATCCTAAAAGTGATCTCACAGTTGTGTTTA NGG 10 8 17 8 24 0 100 5 10