Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164547_4d2x GCCCTATATCATAGCCCCCATCCAACTTGCAG(+AGGACA)GTACCTGTTCCAGGAGCCACTGGGACAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613164546_rzvx TAATGATCTTAAACATACAAAAACATGGTTTG(+AGGACA)GTACCTGAGTCATTTTTAGAGTTTATGCTCA NGG 10 8 17 8 24 0 100 5 10
    240613164544_v724 TTACTTGAGCATAAACTCTAAAAATGACTCAG(+AGGACA)GTACCAAACCATGTTTTTGTATGTTTAAGAT NGG 10 8 17 8 24 0 100 5 10
    240613164543_j8dr GAATGTTAGATATTTTTCCCATGGGGTCAAAG(+AGGACA)GTACCAAAGTATATGATTGTGAGTGGAAACA NGG 10 8 17 8 24 0 100 5 10
    240613164542_2of3 CCCTATGTTTCCACTCACAATCATATACTTTG(+AGGACA)GTACCTTTGACCCCATGGGAAAAATATCTAA NGG 10 8 17 8 24 0 100 5 10
    240613164540_zdx8 GACATTCTAGGTTTGATCTCCAACAACGGATG(+AGGACA)GTACCAATTCATTGTGTGAAGACTCCTTCAC NGG 10 8 17 8 24 0 100 5 10
    240613164539_683n TCCCGGTGAAGGAGTCTTCACACAATGAATTG(+AGGACA)GTACCATCCGTTGTTGGAGATCAAACCTAGA NGG 10 8 17 8 24 0 100 5 10
    240613164538_m1jg GGAGCAATGAGTCATGGTGCCTCTGGCTTCTG(+AGGACA)GTACCCCTTACAACAAGAATTCCACCCAGGC NGG 10 8 17 8 24 0 100 5 10
    240613164536_f4co TGCTGGCCTGGGTGGAATTCTTGTTGTAAGGG(+AGGACA)GTACCAGAAGCCAGAGGCACCATGACTCATT NGG 10 8 17 8 24 0 100 5 10
    240613164534_uy8k CTTCCTGACAAGAAGCGTGTGCTTGCAGGAAG(+AGGACA)GTACCACGCACTTTCTGACTGCAGCCCTTCC NGG 10 8 17 8 24 0 100 5 10