Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164648_w842 TCCAGACCTCAAATCGTCAATGTCCACCTTTG(+AGGACA)GATCCTTGTTTTCAACATCACAGCTGGTGAG NGG 10 8 17 8 24 0 100 5 10
    240613164645_5bzt GTTAAATATATCTACTCTTTTTATATTCCTGG(+AGGACA)GATCCAGGATGAAAAAAAATTTAAATATACA NGG 10 8 17 8 24 0 100 5 10
    240613164643_ce5u TCAGATGTATATTTAAATTTTTTTTCATCCTG(+AGGACA)GATCCCAGGAATATAAAAAGAGTAGATATAT NGG 10 8 17 8 24 0 100 5 10
    240613164642_vy48 TCAAGGAAAATTGATTTGAAGTATAGACACTG(+AGGACA)GATCCCCAATCTCTTAAAAATTGCTTCTATT NGG 10 8 17 8 24 0 100 5 10
    240613164640_1rol TTGTTAATAGAAGCAATTTTTAAGAGATTGGG(+AGGACA)GATCCAGTGTCTATACTTCAAATCAATTTTC NGG 10 8 17 8 24 0 100 5 10
    240613164638_bz1n AGCAGGTTAAATTATCTTAACATCTTTGAATG(+AGGACA)GTACCTAAGATTTTTCTGAGCACTGTACTGG NGG 10 8 17 8 24 0 100 5 10
    240613164637_jf5m TGATACCAGTACAGTGCTCAGAAAAATCTTAG(+AGGACA)GTACCATTCAAAGATGTTAAGATAATTTAAC NGG 10 8 17 8 24 0 100 5 10
    240613164635_5l3k CTATGGGTTAAATGGCCCAGCTCTCTTGCCAG(+AGGACA)GATCCATTTCATTTCCTATAGCGCAGTTAGC NGG 10 8 17 8 24 0 100 5 10
    240613164634_x9yy GGTGTGCTAACTGCGCTATAGGAAATGAAATG(+AGGACA)GATCCTGGCAAGAGAGCTGGGCCATTTAACC NGG 10 8 17 8 24 0 100 5 10
    240613164633_sqgw AAATCCAGAGGGCAAAAGTGTCTGATTAAGTG(+AGGACA)GATCCTGCAAGGCAGCACCCATAAGTGAACC NGG 10 8 17 8 24 0 100 5 10