Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164729_gxn3 GGCAAATCAAAATATAGTGTATTTTGTATTAG(+AGGACA)GTACCGCGAGCACAGCTTAATACATAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240613164727_u5zd CTTTTACTTGCCTGAGTCAACATTTTTAAAGG(+AGGACA)GATCCATTTATTCTTCCTTTGTAGTTTTCGC NGG 10 8 17 8 24 0 100 5 10
    240613164726_569w GTAAAGCGAAAACTACAAAGGAAGAATAAATG(+AGGACA)GATCCCTTTAAAAATGTTGACTCAGGCAAGT NGG 10 8 17 8 24 0 100 5 10
    240613164723_00kj GCTACTGTACTTGCCTCTGCCTGAGCACCAGG(+AGGACA)GATCCAAATCATTGTATAACACACCTCCTGG NGG 10 8 17 8 24 0 100 5 10
    240613164722_gv0f TCTCTCCAGGAGGTGTGTTATACAATGATTTG(+AGGACA)GATCCCTGGTGCTCAGGCAGAGGCAAGTACA NGG 10 8 17 8 24 0 100 5 10
    240613164721_77wz CTCCTCGTGTTCTGTTAGAACTCAAGGACAAG(+AGGACA)GTACCACAGAGAACACCTGCAATGTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240613164719_lyq1 GTGACAAAGAAACATTGCAGGTGTTCTCTGTG(+AGGACA)GTACCTTGTCCTTGAGTTCTAACAGAACACG NGG 10 8 17 8 24 0 100 5 10
    240613164718_5l6p CCACCTGAGAGCAGGGACAACAGAACAGACAG(+AGGACA)GTACCCATCAGTTCTGCTCCAGCAAGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240613164716_3k9g CAGTAGCTTGCTTGCTGGAGCAGAACTGATGG(+AGGACA)GTACCTGTCTGTTCTGTTGTCCCTGCTCTCA NGG 10 8 17 8 24 0 100 5 10
    240613164715_6a5c ATCCTGGGTCCTACCCAGGCAGTGGAAATGAG(+AGGACA)GTACCATTTTTAAAGTACTTGTGTGAAATTA NGG 10 8 17 8 24 0 100 5 10