Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164801_a43y ATTCTACTCTTTAAGTACAATGATTTTGTTTG(+AGGACA)GTACCAACTAATTTAAGTCATTGAAAAAATA NGG 10 8 17 8 24 0 100 5 10
    240613164759_8dmb AGTAAGGGGTCAGTGTGCTGATGGTTTTCAGG(+AGGACA)GTACCGGGCGCGCTGTGTTGGCTGTGCGGGG NGG 10 8 17 8 24 0 100 5 10
    240613164757_wqvn CCGAACCCCGCACAGCCAACACAGCGCGCCCG(+AGGACA)GTACCCTGAAAACCATCAGCACACTGACCCC NGG 10 8 17 8 24 0 100 5 10
    240613164756_w37r AACCAACTTTATCTAAGGTGCTGCTGGCAGTG(+AGGACA)GATCCTAAGATCTTTTGAGCATGACCCTTAG NGG 10 8 17 8 24 0 100 5 10
    240613164754_j0hs TCTCTCTAAGGGTCATGCTCAAAAGATCTTAG(+AGGACA)GATCCACTGCCAGCAGCACCTTAGATAAAGT NGG 10 8 17 8 24 0 100 5 10
    240613164752_hijd CAATCTGCTGCTTGCTTGCGCACCCCTGCAGG(+AGGACA)GTACCCCCCTCCCCACCAAATGGGATCTCAT NGG 10 8 17 8 24 0 100 5 10
    240613164750_hhwr ACAAGATGAGATCCCATTTGGTGGGGAGGGGG(+AGGACA)GTACCCTGCAGGGGTGCGCAAGCAAGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240613164749_kw7m CTTGTGGCCTCCACAGGCACTGAATGCACATG(+AGGACA)GTACCTAGACATACTCAGGCAAACACTCATA NGG 10 8 17 8 24 0 100 5 10
    240613164747_gifb ATGTGTATGAGTGTTTGCCTGAGTATGTCTAG(+AGGACA)GTACCATGTGCATTCAGTGCCTGTGGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240613164746_1bpb GCACATACGCTCACGTGTATCTTCTTTTGAAG(+AGGACA)GTACCTTCTTTACCTAGCAGTTTAACACATC NGG 10 8 17 8 24 0 100 5 10