Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164848_rawv ACTTTCACGGTATGAGAGTCAAAGGGCAGGAG(+AGGACA)GTACCACCGTTTCTGGTAGTGACAGGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613164847_u6ww CTAATGCAGCCCAGTAGAGTGGAGCTCAGGAG(+AGGACA)GTACCCACAGACGGGATTTCTCTTGGTCTTG NGG 10 8 17 8 24 0 100 5 10
    240613164845_29ej TCAGTCAAGACCAAGAGAAATCCCGTCTGTGG(+AGGACA)GTACCTCCTGAGCTCCACTCTACTGGGCTGC NGG 10 8 17 8 24 0 100 5 10
    240613164842_w85y GCGCCAGTCCTCCGATAGACTGCGTCGCCCGG(+AGGACA)GTACCCGTGTTCCCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613164840_oa8h GCAAACAGCAAGAGGCTTTATTGGGAACACGG(+AGGACA)GTACCCGGGCGACGCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10
    240613164839_iw7m TAGAAGCAGGTTCTTGGAAGTTACAATTTAAG(+AGGACA)GTACCCCCAAAAAGTTGGAAATAAAAGGAAA NGG 10 8 17 8 24 0 100 5 10
    240613164836_0nma ATTTGTTTCCTTTTATTTCCAACTTTTTGGGG(+AGGACA)GTACCTTAAATTGTAACTTCCAAGAACCTGC NGG 10 8 17 8 24 0 100 5 10
    240613164835_dsx0 ACATACACAGCTACAGAGTGAGAGTTGGTTTG(+AGGACA)GATCCTCTTCATTTCATTTGTTTGCCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613164834_o1zn ATAACAGAAAGGCAAACAAATGAAATGAAGAG(+AGGACA)GATCCAAACCAACTCTCACTCTGTAGCTGTG NGG 10 8 17 8 24 0 100 5 10
    240613164831_2eqa CTCTTGTGTGTCCTGCCATCAGTGTGACCAGG(+AGGACA)GTACCTCAATAAAGAGTGGTCGAGGAAAGGT NGG 10 8 17 8 24 0 100 5 10