Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164951_vnm2 CCTGGGGCACCTGTTTCTCTTTTCATTTCACG(+AGGACA)GATCCCTGAAGGAGGTCTAGAAGAAAAGGCG NGG 10 8 17 8 24 0 100 5 10
    240613164950_xqhm CCATCAGTTGTTTTAACAAACGTAGGTGATCG(+AGGACA)GTACCTAGAAACTGTTATTTCAGTTAGACAT NGG 10 8 17 8 24 0 100 5 10
    240613164949_okg6 TTGTCATGTCTAACTGAAATAACAGTTTCTAG(+AGGACA)GTACCGATCACCTACGTTTGTTAAAACAACT NGG 10 8 17 8 24 0 100 5 10
    240613164947_v00u CTGATGCCTGTGGAGGCCAGGAGTAAATGTTG(+AGGACA)GATCCTTTGGAACTAGAGTTACAGACAGTTG NGG 10 8 17 8 24 0 100 5 10
    240613164945_bj2k ACTCACAACTGTCTGTAACTCTAGTTCCAAAG(+AGGACA)GATCCAACATTTACTCCTGGCCTCCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240613164944_b1tw CTGGACACACAGAGTCAAAGTAGGAAAACATG(+AGGACA)GATCCCTGAGACCTTCACAGCGACAGACTAG NGG 10 8 17 8 24 0 100 5 10
    240613164942_ypnj CTGTGCTAGTCTGTCGCTGTGAAGGTCTCAGG(+AGGACA)GATCCATGTTTTCCTACTTTGACTCTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240613164940_evuy CTTTAAAGTATTTCATAATACCTTGAGGATGG(+AGGACA)GATCCAATTCTGAACACAAGGTCCGCTTATA NGG 10 8 17 8 24 0 100 5 10
    240613164939_povj AGAAATATAAGCGGACCTTGTGTTCAGAATTG(+AGGACA)GATCCCATCCTCAAGGTATTATGAAATACTT NGG 10 8 17 8 24 0 100 5 10
    240613164938_qfwm CGAGCCCTCTAAGTTGGCGTTTGCTACTAAAG(+AGGACA)GATCCCCAGAATCCCCAATACTGTAACAACG NGG 10 8 17 8 24 0 100 5 10