Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165008_twb0 CCTGGCATGGTGTGTTGCCTCCTGTGGACCAG(+AGGACA)GATCCACAAGTCAAAATACAGCTTTCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240613165006_ypse AATAATGCTACATTGAACAGGGATCGGGGTTG(+AGGACA)GTACCTGTAAACATTGATCCCACTGCATCCG NGG 10 8 17 8 24 0 100 5 10
    240613165004_wcco CGTAACGGATGCAGTGGGATCAATGTTTACAG(+AGGACA)GTACCAACCCCGATCCCTGTTCAATGTAGCA NGG 10 8 17 8 24 0 100 5 10
    240613165003_vbip TAAGGTAGCCCTGGTCTGGAATTTTGAAACAG(+AGGACA)GATCCAAGCAAGTGTCTCCTTCATTTGCACA NGG 10 8 17 8 24 0 100 5 10
    240613165001_e0hj AGCTGTGTGCAAATGAAGGAGACACTTGCTTG(+AGGACA)GATCCTGTTTCAAAATTCCAGACCAGGGCTA NGG 10 8 17 8 24 0 100 5 10
    240613164959_h9gr TGATTTACCTTCAGTGTCCTGGGTGAGGTGGG(+AGGACA)GTACCATCATTATGACCCAGGAAAAGGGACA NGG 10 8 17 8 24 0 100 5 10
    240613164958_v1vl TACCGTGTCCCTTTTCCTGGGTCATAATGATG(+AGGACA)GTACCCCACCTCACCCAGGACACTGAAGGTA NGG 10 8 17 8 24 0 100 5 10
    240613164956_37os GTGTGTCATTGCCCTCCCCTGTGGTCATCTGG(+AGGACA)GTACCCCTGCCCATCCATTGTTTTTAGAACA NGG 10 8 17 8 24 0 100 5 10
    240613164954_711t AGGGTTGTTCTAAAAACAATGGATGGGCAGGG(+AGGACA)GTACCCAGATGACCACAGGGGAGGGCAATGA NGG 10 8 17 8 24 0 100 5 10
    240613164953_fmsk CTCATCGCCTTTTCTTCTAGACCTCCTTCAGG(+AGGACA)GATCCGTGAAATGAAAAGAGAAACAGGTGCC NGG 10 8 17 8 24 0 100 5 10