Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165110_xf5i TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+AGGACA)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240613165108_7tn8 GACCTCCTCCTTGCAAAAGCTGGAAGGGATGG(+AGGACA)GTACCCCTCTTATGACCCATGAGAGGCCTTA NGG 10 8 17 8 24 0 100 5 10
    240613165106_ugnb GTCATTAAGGCCTCTCATGGGTCATAAGAGGG(+AGGACA)GTACCCATCCCTTCCAGCTTTTGCAAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613165105_jdux CTGTTGCCTAAATAGTGGCAATGTTTCGCAGG(+AGGACA)GTACCAGTGATGATATTGGGGATCAAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240613165103_0lhy TTACATCACCTTGATCCCCAATATCATCACTG(+AGGACA)GTACCCTGCGAAACATTGCCACTATTTAGGC NGG 10 8 17 8 24 0 100 5 10
    240613165102_r8ax GAGGCCTGATACACAGGAGGCTGAGGCAGAAG(+AGGACA)GATCCAAGGTCAAGGCCTGCCAGACAGGTAC NGG 10 8 17 8 24 0 100 5 10
    240613165100_tlyi ACTCTGTACCTGTCTGGCAGGCCTTGACCTTG(+AGGACA)GATCCTTCTGCCTCAGCCTCCTGTGTATCAG NGG 10 8 17 8 24 0 100 5 10
    240613165058_m42p GGCTTCAGAGGAGACTGTGGCAACATCATGTG(+AGGACA)GTACCAACAGGGCAGTGTTTTCAGTATGTGC NGG 10 8 17 8 24 0 100 5 10
    240613165057_9vy7 CGTGTGCACATACTGAAAACACTGCCCTGTTG(+AGGACA)GTACCACATGATGTTGCCACAGTCTCCTCTG NGG 10 8 17 8 24 0 100 5 10
    240613165055_615q GTAGGCTTTTATATAAGGCAAAAAGCAAGATG(+AGGACA)GATCCACTACTTTCCACAGAAAGAAACAGCT NGG 10 8 17 8 24 0 100 5 10