Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165318_cq52 GGCCACCGACACTGGGTAGGGATAGCCACGGG(+AGGACA)GTACCTGGTGTAATGTCCCTACCAGCTGACT NGG 10 8 17 8 24 0 100 5 10
    240613165316_ucz8 CTCTCAGTCAGCTGGTAGGGACATTACACCAG(+AGGACA)GTACCCCGTGGCTATCCCTACCCAGTGTCGG NGG 10 8 17 8 24 0 100 5 10
    240613165315_8ol0 CATAACAACTGCAGGTGCTCAGACCACTGTTG(+AGGACA)GTACCAGAACCAGTGCCTTGGTGGGGCCATC NGG 10 8 17 8 24 0 100 5 10
    240613165313_9s2p GGCAAGATGGCCCCACCAAGGCACTGGTTCTG(+AGGACA)GTACCAACAGTGGTCTGAGCACCTGCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240613165312_nyg7 TACTGTACTGGGTTCCGTGTGTCACTGGGGTG(+AGGACA)GTACCTATATTTTAATACTGTTCCTAACATT NGG 10 8 17 8 24 0 100 5 10
    240613165310_jhj9 AATGAAATGTTAGGAACAGTATTAAAATATAG(+AGGACA)GTACCACCCCAGTGACACACGGAACCCAGTA NGG 10 8 17 8 24 0 100 5 10
    240613165309_13pz CTTCCATGAACTCTTTAGGCCATGATTCTGTG(+AGGACA)GTACCTAGTTATGATAGTACAAATCAGATCA NGG 10 8 17 8 24 0 100 5 10
    240613165308_laq8 TTCTCTGATCTGATTTGTACTATCATAACTAG(+AGGACA)GTACCACAGAATCATGGCCTAAAGAGTTCAT NGG 10 8 17 8 24 0 100 5 10
    240613165306_hvle AGTTTTTACACCATGGAAATGGGGTACACGGG(+AGGACA)GTACCCAGGTCATCAGCATGCCCTCTAAAGT NGG 10 8 17 8 24 0 100 5 10
    240613165304_bbc5 TGGGTACTTTAGAGGGCATGCTGATGACCTGG(+AGGACA)GTACCCCGTGTACCCCATTTCCATGGTGTAA NGG 10 8 17 8 24 0 100 5 10