Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165231_wipx TCTTCCTCACCTCCCAGGTTCATAGGTCAAAG(+AGGACA)GTACCTAGTACCATGGGGGGATGCCAAGGGA NGG 10 8 17 8 24 0 100 5 10
    240613165229_ljj2 CCCCCAGGATTGGAATGGCTGAAAAGGGAGGG(+AGGACA)GATCCATCTCTTTCCCATAGTTTTGGGACCT NGG 10 8 17 8 24 0 100 5 10
    240613165228_fyhw TTATTAGGTCCCAAAACTATGGGAAAGAGATG(+AGGACA)GATCCCCTCCCTTTTCAGCCATTCCAATCCT NGG 10 8 17 8 24 0 100 5 10
    240613165226_6rde TAGAGTATTCACTAAAGGTGGATGTGCCTCAG(+AGGACA)GTACCACAGTAACGCAGTCTTCCAAGTTAAC NGG 10 8 17 8 24 0 100 5 10
    240613165224_wo4h GAGCTGTTAACTTGGAAGACTGCGTTACTGTG(+AGGACA)GTACCTGAGGCACATCCACCTTTAGTGAATA NGG 10 8 17 8 24 0 100 5 10
    240613165222_wia6 AGAGCACATTCTTAGCATGCACAGAACCTTAG(+AGGACA)GATCCATCGCCAGCAACACACACAATGAAAA NGG 10 8 17 8 24 0 100 5 10
    240613165221_ld4j TTTGTTTTTCATTGTGTGTGTTGCTGGCGATG(+AGGACA)GATCCTAAGGTTCTGTGCATGCTAAGAATGT NGG 10 8 17 8 24 0 100 5 10
    240613165219_x3xp CTCCAGAGTGCCCTGCAGCAATGCCCAGGAGG(+AGGACA)GATCCAGACGGCTCCATTTGAGCTAAGCAGC NGG 10 8 17 8 24 0 100 5 10
    240613165218_0sgu CCCTGGCTGCTTAGCTCAAATGGAGCCGTCTG(+AGGACA)GATCCCTCCTGGGCATTGCTGCAGGGCACTC NGG 10 8 17 8 24 0 100 5 10
    240613165217_cuoz TAAAGTTCAGACCTGGGGTGGGAGAGAAGAGG(+AGGACA)GTACCGGAATGATTATTAATGTACAAATGCA NGG 10 8 17 8 24 0 100 5 10