Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165244_4pyu CTCTTATTACTCCCCTGCCCCCCACAGAGCAG(+AGGACA)GATCCAATTTACCTGGGGGGAGGGGGAGAGA NGG 10 8 17 8 24 0 100 5 10
    240613165243_lx5y GTCAGCTGCAGGGGGATCTGGGTAAGGAAAAG(+AGGACA)GTACCGCTCTCCACAATCTCCTGTCTTTAAG NGG 10 8 17 8 24 0 100 5 10
    240613165242_3rii TCCAACTTAAAGACAGGAGATTGTGGAGAGCG(+AGGACA)GTACCTTTTCCTTACCCAGATCCCCCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240613165240_0p4r TTTGTTTTGTTTTTTTTAACCCTCAAATTAGG(+AGGACA)GTACCCTAATCAAGGCAAAAAACTTAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240613165238_g7ph AGCCATTTCTTAAGTTTTTTGCCTTGATTAGG(+AGGACA)GTACCCTAATTTGAGGGTTAAAAAAAACAAA NGG 10 8 17 8 24 0 100 5 10
    240613165237_426q GAGAATTTTATATAAAAGTAACAGAAATAAAG(+AGGACA)GTACCAAAAAAGAAAAAAAGGAAAAAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240613165236_mp7q TTGTTTTTCTTTTTTCCTTTTTTTCTTTTTTG(+AGGACA)GTACCTTTATTTCTGTTACTTTTATATAAAA NGG 10 8 17 8 24 0 100 5 10
    240613165235_x91l TTTCAAGTGTCATTGCAATTTATTACTATTAG(+AGGACA)GTACCAAGTGGGTGGGAATAATATACAGCAT NGG 10 8 17 8 24 0 100 5 10
    240613165234_6uxc CACAAATGCTGTATATTATTCCCACCCACTTG(+AGGACA)GTACCTAATAGTAATAAATTGCAATGACACT NGG 10 8 17 8 24 0 100 5 10
    240613165232_3y4d GGGCTTCCCTTGGCATCCCCCCATGGTACTAG(+AGGACA)GTACCTTTGACCTATGAACCTGGGAGGTGAG NGG 10 8 17 8 24 0 100 5 10