Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165334_ufoa CTCAGTTGGCAGAGTGCTAGCCTAGATGCATG(+AGGACA)GATCCTTGGGCTCAGGCCTCAGCACTGAACT NGG 10 8 17 8 24 0 100 5 10
    240613165332_b65n TGCCTAGTTCAGTGCTGAGGCCTGAGCCCAAG(+AGGACA)GATCCATGCATCTAGGCTAGCACTCTGCCAA NGG 10 8 17 8 24 0 100 5 10
    240613165330_vwzp CCACCCCACCACAGAATGCTGATATGCCAGTG(+AGGACA)GTACCCAGTACCTCTTGTATATAGTTATTGC NGG 10 8 17 8 24 0 100 5 10
    240613165329_3khj TATTTGCAATAACTATATACAAGAGGTACTGG(+AGGACA)GTACCACTGGCATATCAGCATTCTGTGGTGG NGG 10 8 17 8 24 0 100 5 10
    240613165327_013a ATGCTGTTCACATGGTGGGTATAACTTTCAAG(+AGGACA)GTACCACCCTGGCCTGCACCTGCCGGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240613165326_jffb TGGGTCCTGACCGGCAGGTGCAGGCCAGGGTG(+AGGACA)GTACCTTGAAAGTTATACCCACCATGTGAAC NGG 10 8 17 8 24 0 100 5 10
    240613165323_fxa4 CTCTTCAAGCCCTCCTTTCAGTGGGGCCCTGG(+AGGACA)GTACCATGATGGCCTCCCTGTCTTCTAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613165322_q4ic GACACTGCCTAGAAGACAGGGAGGCCATCATG(+AGGACA)GTACCCAGGGCCCCACTGAAAGGAGGGCTTG NGG 10 8 17 8 24 0 100 5 10
    240613165321_0zjl ACCCTAAAGTTTGGAATGGGAACATGTGCATG(+AGGACA)GATCCAACAACCAACCCAACCCTCAAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240613165319_blz8 GGACCGGAACTTGAGGGTTGGGTTGGTTGTTG(+AGGACA)GATCCATGCACATGTTCCCATTCCAAACTTT NGG 10 8 17 8 24 0 100 5 10