Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165143_8xgf CAAGTCAAAGCAGAACACATGTTGTAAACTTG(+AGGACA)GATCCAAGGATTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240613165142_as11 CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+AGGACA)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240613165140_6gp8 TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+AGGACA)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240613165138_1spo TGGAGATCAGTCAACAAAATTGTAAATCCTTG(+AGGACA)GATCCAAGTTTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240613165137_ggpo CAAGTCAAAGCAGAACACATGTTGTAAACTTG(+AGGACA)GATCCAAGGATTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240613165135_7cw0 CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+AGGACA)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240613165133_ygit TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+AGGACA)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240613165132_3506 CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+AGGACA)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240613165130_5pou TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+AGGACA)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240613165129_l94w CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+AGGACA)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10