Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165054_dd8d TATAGAGCTGTTTCTTTCTGTGGAAAGTAGTG(+AGGACA)GATCCATCTTGCTTTTTGCCTTATATAAAAG NGG 10 8 17 8 24 0 100 5 10
    240613165052_e535 TGGTTGTTTGAATGCAGGTGGGCTGAGGTGGG(+AGGACA)GATCCCCGAGAAAGGGTGCGTGCCACGGAGA NGG 10 8 17 8 24 0 100 5 10
    240613165050_mjiv GCTTCTCTCCGTGGCACGCACCCTTTCTCGGG(+AGGACA)GATCCCCACCTCAGCCCACCTGCATTCAAAC NGG 10 8 17 8 24 0 100 5 10
    240613165049_d4pp GAGCTCCATTAAAGTCTCATGACATGCAAGTG(+AGGACA)GATCCTCAGAGCAGATCCCCGTGAGGCATAG NGG 10 8 17 8 24 0 100 5 10
    240613165048_qze2 TCCCTCTATGCCTCACGGGGATCTGCTCTGAG(+AGGACA)GATCCACTTGCATGTCATGAGACTTTAATGG NGG 10 8 17 8 24 0 100 5 10
    240613165046_24mv CCATAAACCTGGAATATGTGTTTATCAGTAGG(+AGGACA)GTACCCTACTTGAGCAGGAACCCTGAATAAA NGG 10 8 17 8 24 0 100 5 10
    240613165044_dm6m ACCAATTTATTCAGGGTTCCTGCTCAAGTAGG(+AGGACA)GTACCCTACTGATAAACACATATTCCAGGTT NGG 10 8 17 8 24 0 100 5 10
    240613165041_bo81 AGAACTTGTAGCACCTTTAACAGATTCCAAGG(+AGGACA)GATCCAGTGTCTTCTCTTGGTTTCTTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240613165040_6cbo GAAATGCCTGAAGAAACCAAGAGAAGACACTG(+AGGACA)GATCCCTTGGAATCTGTTAAAGGTGCTACAA NGG 10 8 17 8 24 0 100 5 10
    240613165038_tdjh CCTAGCATTGCTGATTGTTTCACACAAGGTAG(+AGGACA)GATCCGTTAGCAATTAAGTGCAACGACTCAT NGG 10 8 17 8 24 0 100 5 10