Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165037_930x CCAAAATGAGTCGTTGCACTTAATTGCTAACG(+AGGACA)GATCCTACCTTGTGTGAAACAATCAGCAATG NGG 10 8 17 8 24 0 100 5 10
    240613165036_4j5x AAAACACTAAGAATGCATTTTAGCCAGGTATG(+AGGACA)GTACCAGGAAAGAGATGGCACATAAAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240613165034_g48k TCTGGGTGCCTTTATGTGCCATCTCTTTCCTG(+AGGACA)GTACCATACCTGGCTAAAATGCATTCTTAGT NGG 10 8 17 8 24 0 100 5 10
    240613165032_nkk4 ATTCCTAAATTTAAAATGGGTGCTTGTCTTAG(+AGGACA)GATCCTCCTATCAAGATACCAGTGAGGAACC NGG 10 8 17 8 24 0 100 5 10
    240613165031_yhoi TCGGAGGTTCCTCACTGGTATCTTGATAGGAG(+AGGACA)GATCCTAAGACAAGCACCCATTTTAAATTTA NGG 10 8 17 8 24 0 100 5 10
    240613165030_hz0q TTAGATCACGGGTGACACTCGGTGACCTTGTG(+AGGACA)GATCCCATAAGGTGGAAAGAGTATTTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240613165028_geh6 TCACACTGGAAAATACTCTTTCCACCTTATGG(+AGGACA)GATCCACAAGGTCACCGAGTGTCACCCGTGA NGG 10 8 17 8 24 0 100 5 10
    240613165026_q9m0 ATTCTGCCATGTGGCTGATTACTTGTGCTCAG(+AGGACA)GTACCATATGTCTGTTTCCTCACCTCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240613165025_thpv CTCCCAGGAAGAGGTGAGGAAACAGACATATG(+AGGACA)GTACCTGAGCACAAGTAATCAGCCACATGGC NGG 10 8 17 8 24 0 100 5 10
    240613165024_c2no AGAATGTGAGGCAGTGCTCAGGTACTGATGAG(+AGGACA)GTACCTGAAGCAGTAAAGGAGTGTGGAGTGT NGG 10 8 17 8 24 0 100 5 10