Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165215_d7jq AGAATTGCATTTGTACATTAATAATCATTCCG(+AGGACA)GTACCCTCTTCTCTCCCACCCCAGGTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240613165213_8s37 TGGAGATCAGTCAACAAATTTGTAAATCCTTG(+AGGACA)GATCCAAGTTTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240613165212_wwso CAAGTCAAAGCAGAACACATGTTGTAAACTTG(+AGGACA)GATCCAAGGATTTACAAATTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240613165211_dr5a TTTGTGTGTTTTGGATCTCATGGCAGTAATAG(+AGGACA)GTACCCGAGGTGTTAACTACCGTTTCTGTAA NGG 10 8 17 8 24 0 100 5 10
    240613165208_pfw0 TGCTGTTACAGAAACGGTAGTTAACACCTCGG(+AGGACA)GTACCTATTACTGCCATGAGATCCAAAACAC NGG 10 8 17 8 24 0 100 5 10
    240613165206_s4md TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+AGGACA)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240613165205_sk4o CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+AGGACA)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240613165203_q580 TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+AGGACA)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240613165202_rekf CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+AGGACA)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240613165200_2lba TGGAGATCAGTCAACAAAATTGTAAATCCTTG(+AGGACA)GATCCAAGTTTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10