Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164903_zrm3 GACATATAGGCTACAGATATATACATTAGTTG(+AGGACA)GATCCACTGTTAAAACTAGATGCAGTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240613164902_lunn CTGGTGACCAAGGAGGCCAGAAAATGGCATTG(+AGGACA)GATCCCCTGAAACTGGAATTTTGGATGGCTA NGG 10 8 17 8 24 0 100 5 10
    240613164900_x75q GATTATAGCCATCCAAAATTCCAGTTTCAGGG(+AGGACA)GATCCAATGCCATTTTCTGGCCTCCTTGGTC NGG 10 8 17 8 24 0 100 5 10
    240613164859_4qx9 ATCTAACATGCTTTTTAACTTGTAGTACAATG(+AGGACA)GATCCAAGTCCAAGAACACAGGTTACATGCA NGG 10 8 17 8 24 0 100 5 10
    240613164857_leqw CACGATGCATGTAACCTGTGTTCTTGGACTTG(+AGGACA)GATCCATTGTACTACAAGTTAAAAAGCATGT NGG 10 8 17 8 24 0 100 5 10
    240613164856_xci6 AGCTGACAACCACACGCAGCTCAAGCTCACGG(+AGGACA)GATCCAATGTCCTCTTCTGTCAGCCACGCAC NGG 10 8 17 8 24 0 100 5 10
    240613164854_qjoe TGGGGGTGCGTGGCTGACAGAAGAGGACATTG(+AGGACA)GATCCCGTGAGCTTGAGCTGCGTGTGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240613164853_wu1h ACTCCAGGGAAAGGAGCGTGTGGGGAGGGAAG(+AGGACA)GTACCAGAAGCTCATCTGATTGGCCCAACGG NGG 10 8 17 8 24 0 100 5 10
    240613164851_3uiw CTAAACCGTTGGGCCAATCAGATGAGCTTCTG(+AGGACA)GTACCTTCCCTCCCCACACGCTCCTTTCCCT NGG 10 8 17 8 24 0 100 5 10
    240613164850_057g TTTTTAAAAACCTGTCACTACCAGAAACGGTG(+AGGACA)GTACCTCCTGCCCTTTGACTCTCATACCGTG NGG 10 8 17 8 24 0 100 5 10