Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164830_3kk5 GAGAAACCTTTCCTCGACCACTCTTTATTGAG(+AGGACA)GTACCCTGGTCACACTGATGGCAGGACACAC NGG 10 8 17 8 24 0 100 5 10
    240613164829_2tj2 ACCTAACTGTGCAGGCTGACGATTAATTACTG(+AGGACA)GATCCAAGAGAACAATACATTCTTATGTACA NGG 10 8 17 8 24 0 100 5 10
    240613164827_10lz TCTTGTGTACATAAGAATGTATTGTTCTCTTG(+AGGACA)GATCCAGTAATTAATCGTCAGCCTGCACAGT NGG 10 8 17 8 24 0 100 5 10
    240613164826_mur6 GGTTCTATATAGCCATATAATATTTTGATATG(+AGGACA)GTACCTTGCAACACTGCATGTTCTTGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240613164825_ty5u TGTTTTCTACCAAGAACATGCAGTGTTGCAAG(+AGGACA)GTACCATATCAAAATATTATATGGCTATATA NGG 10 8 17 8 24 0 100 5 10
    240613164823_4yl4 AGCTGCCATGAGGATTCTGGGAATTGAATTTG(+AGGACA)GATCCTCTGGACAAGTGACCAGTACTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240613164821_2rtm AGCAGTTAAGAGTACTGGTCACTTGTCCAGAG(+AGGACA)GATCCAAATTCAATTCCCAGAATCCTCATGG NGG 10 8 17 8 24 0 100 5 10
    240613164820_jece GTGCTGTGTTCTGGAGAGATCTCACGTGGATG(+AGGACA)GTACCGGTGCTCTGAGCACCACAGATGTGAG NGG 10 8 17 8 24 0 100 5 10
    240613164818_e30s TGTAGCTCACATCTGTGGTGCTCAGAGCACCG(+AGGACA)GTACCATCCACGTGAGATCTCTCCAGAACAC NGG 10 8 17 8 24 0 100 5 10
    240613164816_d37l TCAAGGAACGTGCACCAAGAAGGCAGCTTGAG(+AGGACA)GATCCTGACACTGAACTGAAGCGCGGGCATT NGG 10 8 17 8 24 0 100 5 10