Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164702_k2dj AATTTAAATTTTCTTTCATATAAAGAGGGCCG(+AGGACA)GTACCTGTCAAAGTGCTATTTTATGCAGTAA NGG 10 8 17 8 24 0 100 5 10
    240613164701_mpbo TTGTAGTTGCCCTGAACCAATCTCTATTCTAG(+AGGACA)GTACCGTGACACCAGTTGAAACAGACCCACC NGG 10 8 17 8 24 0 100 5 10
    240613164700_7lsr AGGACGGTGGGTCTGTTTCAACTGGTGTCACG(+AGGACA)GTACCTAGAATAGAGATTGGTTCAGGGCAAC NGG 10 8 17 8 24 0 100 5 10
    240613164659_j3fl GCATTTGATGTTTGTAAATAAATCCACAGTAG(+AGGACA)GATCCAAGCTGAAGAGGTGATACATGGTCAG NGG 10 8 17 8 24 0 100 5 10
    240613164657_mrt7 CTAGGCTGACCATGTATCACCTCTTCAGCTTG(+AGGACA)GATCCTACTGTGGATTTATTTACAAACATCA NGG 10 8 17 8 24 0 100 5 10
    240613164656_98h3 ATCCATGATGACTTCCAAGGACTGTGTGTTAG(+AGGACA)GTACCCGAAAAAGCTCCAAAGCTTGAATCAG NGG 10 8 17 8 24 0 100 5 10
    240613164654_uys5 ATAGTCTGATTCAAGCTTTGGAGCTTTTTCGG(+AGGACA)GTACCTAACACACAGTCCTTGGAAGTCATCA NGG 10 8 17 8 24 0 100 5 10
    240613164653_i1kj TGCTAAGCATGATCTAGAATAGAAGCCACTGG(+AGGACA)GTACCCCTAACTATATTTAGTGATTTAGAAC NGG 10 8 17 8 24 0 100 5 10
    240613164651_tqy1 GCTGAGTTCTAAATCACTAAATATAGTTAGGG(+AGGACA)GTACCCAGTGGCTTCTATTCTAGATCATGCT NGG 10 8 17 8 24 0 100 5 10
    240613164649_r1sw CAGTGCTCACCAGCTGTGATGTTGAAAACAAG(+AGGACA)GATCCAAAGGTGGACATTGACGATTTGAGGT NGG 10 8 17 8 24 0 100 5 10