Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164601_0fgi TAAGCAAGCTGAGTTTATGAACACATTTTGAG(+AGGACA)GATCCTAAGGTAATTACAGTCTCACTGGGGT NGG 10 8 17 8 24 0 100 5 10
    240613164559_ul9f CCTTTGTATCCTCATGGTTCTTTGACCTTCGG(+AGGACA)GTACCTGAATAGAAAAGGCAATGCTTTTTGT NGG 10 8 17 8 24 0 100 5 10
    240613164558_pk3u ACTTCACAAAAAGCATTGCCTTTTCTATTCAG(+AGGACA)GTACCCGAAGGTCAAAGAACCATGAGGATAC NGG 10 8 17 8 24 0 100 5 10
    240613164556_s2vq AGACACCCCCATGTGACTAGGGAGTGAGTCTG(+AGGACA)GTACCTCACTCAGGATGATAGCATTTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240613164555_4ggl TATCTGAAGGAAATGCTATCATCCTGAGTGAG(+AGGACA)GTACCAGACTCACTCCCTAGTCACATGGGGG NGG 10 8 17 8 24 0 100 5 10
    240613164554_jsuq CCCCTATTTTATCTTTATAGTGATCTCAGTAG(+AGGACA)GTACCCACTATGGTTACTGTCAAACGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240613164552_0179 TCCTTGGCAGCGTTTGACAGTAACCATAGTGG(+AGGACA)GTACCTACTGAGATCACTATAAAGATAAAAT NGG 10 8 17 8 24 0 100 5 10
    240613164551_wgce GAGCACAGGATTCTGGCACAGTGTATATACAG(+AGGACA)GTACCAAAGTCCCTCTCCTAGAATGCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240613164549_vp2j ACTGTTCCCTGCATTCTAGGAGAGGGACTTTG(+AGGACA)GTACCTGTATATACACTGTGCCAGAATCCTG NGG 10 8 17 8 24 0 100 5 10
    240613164548_3ip2 ATTCTTTCCTGTCCCAGTGGCTCCTGGAACAG(+AGGACA)GTACCTGCAAGTTGGATGGGGGCTATGATAT NGG 10 8 17 8 24 0 100 5 10