Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164632_lj1o TCCTGGGTTCACTTATGGGTGCTGCCTTGCAG(+AGGACA)GATCCACTTAATCAGACACTTTTGCCCTCTG NGG 10 8 17 8 24 0 100 5 10
    240613164629_sdt4 CCCCAAGATCTTTGAGATGTCAGAGCTGTGGG(+AGGACA)GTACCTGCTCAGGAAAACCAGCCAAAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240613164628_qoab ATTCTTTCTCTTTGGCTGGTTTTCCTGAGCAG(+AGGACA)GTACCCCACAGCTCTGACATCTCAAAGATCT NGG 10 8 17 8 24 0 100 5 10
    240613164627_0bhz TCTCTTTGGAGGACTGCAGAAGATCATGTGTG(+AGGACA)GATCCAAGACATTGGAGCAAGAAGCTGTAAC NGG 10 8 17 8 24 0 100 5 10
    240613164626_e8c0 TCAGTGTTACAGCTTCTTGCTCCAATGTCTTG(+AGGACA)GATCCACACATGATCTTCTGCAGTCCTCCAA NGG 10 8 17 8 24 0 100 5 10
    240613164624_21ov CCCCAAGATCTTTGAGATGCCAGAGCTGTGGG(+AGGACA)GTACCTGCTCATGAAAACCAGCCAAAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240613164623_jxb0 ATTCTTTCTCTTTGGCTGGTTTTCATGAGCAG(+AGGACA)GTACCCCACAGCTCTGGCATCTCAAAGATCT NGG 10 8 17 8 24 0 100 5 10
    240613164621_hjf7 TCTCTTTGGAGGATAGCAGAAGATCATGTGTG(+AGGACA)GATCCAAGACATTGGAACAAGAAACTGTAAC NGG 10 8 17 8 24 0 100 5 10
    240613164620_6rgq TCAGTGTTACAGTTTCTTGTTCCAATGTCTTG(+AGGACA)GATCCACACATGATCTTCTGCTATCCTCCAA NGG 10 8 17 8 24 0 100 5 10
    240613164619_ur29 CACAGAAGTCATCACATAGGGAAGATATGAAG(+AGGACA)GATCCTTTAGAGAAAACTTTAACAAAGTTTT NGG 10 8 17 8 24 0 100 5 10