Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613164505_hsoo ATGAATAAACACAACTGTGAGATCACTTTTAG(+AGGACA)GATCCCTATCAATAGTTATTTATGTAAGAAC NGG 10 8 17 8 24 0 100 5 10
    240613164504_f9n4 TTTGCTGAGTCAGGATTTATATGGATACAAAG(+AGGACA)GATCCCTTCTGAAAACTGTCCATTGTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240613164503_5c8j GTACGCCTGCACAATGGACAGTTTTCAGAAGG(+AGGACA)GATCCTTTGTATCCATATAAATCCTGACTCA NGG 10 8 17 8 24 0 100 5 10
    240613164501_bmwh GAATAGTGGCTGTTCATTTATACCCTCAGCTG(+AGGACA)GTACCAACTGAGAAGCTTGCTGTCTGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240613164500_pk8v AGGTAGCCAGCAGACAGCAAGCTTCTCAGTTG(+AGGACA)GTACCAGCTGAGGGTATAAATGAACAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240613164459_ogo8 GAAACTATTCCTTTGGAGGAGTCCTGATTGTG(+AGGACA)GATCCCAAATGTTGTAGACTTTGAACTTTGC NGG 10 8 17 8 24 0 100 5 10
    240613164457_hare TAAAAGCAAAGTTCAAAGTCTACAACATTTGG(+AGGACA)GATCCACAATCAGGACTCCTCCAAAGGAATA NGG 10 8 17 8 24 0 100 5 10
    240613164455_simc TCACAACTAAGGTTACCCCCATTGATTCTTGG(+AGGACA)GTACCTCCCCTATCCCATACCTCCCTCATGT NGG 10 8 17 8 24 0 100 5 10
    240613164454_x5mx TGAAGACATGAGGGAGGTATGGGATAGGGGAG(+AGGACA)GTACCCAAGAATCAATGGGGGTAACCTTAGT NGG 10 8 17 8 24 0 100 5 10
    240613164452_9vp2 GTCAGGCCTTTCACACCAATACTCCCCTCCTG(+AGGACA)GTACCAACTTCTTAGGTTGGGTTTTATCACC NGG 10 8 17 8 24 0 100 5 10