Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605120612_tnto ACCTTTACAGACTTGCCCCTTGCCTCACACAG(+CAGTGT)GATCCAATCAAATTTAATTCAAGTCTCAATG NGG 10 8 17 8 24 0 100 5 10
    240605120611_i79j TTTCACATTGAGACTTGAATTAAATTTGATTG(+CAGTGT)GATCCTGTGTGAGGCAAGGGGCAAGTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240605120609_a02p AATGAGTCAGAATTGGCCCTACAGCCCCTTTG(+CAGTGT)GATCCTGCAGGATACCCTAGTGGCTAAACCT NGG 10 8 17 8 24 0 100 5 10
    240605120608_5nvu GGGCTAGGTTTAGCCACTAGGGTATCCTGCAG(+CAGTGT)GATCCAAAGGGGCTGTAGGGCCAATTCTGAC NGG 10 8 17 8 24 0 100 5 10
    240605120607_zrxn TGGGGCAGCTGTCAAAAGCTGCAGTTGGATAG(+CAGTGT)GTACCAGCCTGTGGTGACTGGTAAGTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605120605_a3ev GTTTGCCACTACTTACCAGTCACCACAGGCTG(+CAGTGT)GTACCTATCCAACTGCAGCTTTTGACAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605120604_8zgu GTCCCAGTTTCCACCTTCCTCTGGCCCAGTAG(+CAGTGT)GATCCCCAATCTGACTCTTTCAAGGCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240605120602_icv8 GAATGTCTGAGCCTTGAAAGAGTCAGATTGGG(+CAGTGT)GATCCTACTGGGCCAGAGGAAGGTGGAAACT NGG 10 8 17 8 24 0 100 5 10
    240605120601_oqqj GGTAACCACATTTTTCAACTCCACCCACCATG(+CAGTGT)GTACCCTAGAACCAAGACTGGAATACTGACT NGG 10 8 17 8 24 0 100 5 10
    240605120559_1r6x TCTCCAGTCAGTATTCCAGTCTTGGTTCTAGG(+CAGTGT)GTACCATGGTGGGTGGAGTTGAAAAATGTGG NGG 10 8 17 8 24 0 100 5 10