Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605120509_ycg8 TGAGCCATGCCTCCAGGTAACTGATGGATTTG(+CAGTGT)GTACCTCTCATCCCAGGCTTTTTAAGAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605120508_dwfa CCAAGGCTTTCTTAAAAAGCCTGGGATGAGAG(+CAGTGT)GTACCAAATCCATCAGTTACCTGGAGGCATG NGG 10 8 17 8 24 0 100 5 10
    240605120506_flmr CTGACATCCAATGGGTCACACCCACAAGTTGG(+CAGTGT)GTACCCGTGAGTTCACTCAGGTGTGTCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605120504_vrt1 GGCCTCAAAGACACACCTGAGTGAACTCACGG(+CAGTGT)GTACCCAACTTGTGGGTGTGACCCATTGGAT NGG 10 8 17 8 24 0 100 5 10
    240605120503_qy0v GTCTCCCACTCCTTGCTTGTTGCCTTTAGAAG(+CAGTGT)GTACCAGGTGGGACTGGGGCTGTGGGGCAAG NGG 10 8 17 8 24 0 100 5 10
    240605120501_gfea TCCACCTTGCCCCACAGCCCCAGTCCCACCTG(+CAGTGT)GTACCTTCTAAAGGCAACAAGCAAGGAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605120500_1npf AGGGGAGCTTGTGATTGCTATGCCTTCAACAG(+CAGTGT)GATCCCTCAGGGTCTCATCTTGAAGAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605120458_pd72 AGCCCTCCACTCTTCAAGATGAGACCCTGAGG(+CAGTGT)GATCCTGTTGAAGGCATAGCAATCACAAGCT NGG 10 8 17 8 24 0 100 5 10
    240605120456_5bly GCTTACATTTACTGGCCCTGAAGCTTCCAAAG(+CAGTGT)GATCCGCATAAAAACCTAGAGAAAGAATAAG NGG 10 8 17 8 24 0 100 5 10
    240605120455_xq7p CTTCCCTTATTCTTTCTCTAGGTTTTTATGCG(+CAGTGT)GATCCTTTGGAAGCTTCAGGGCCAGTAAATG NGG 10 8 17 8 24 0 100 5 10