Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605115740_6mbj TCCTTATTCAGTTAGATAGATGTGCCATCCAG(+CAGTGT)GATCCCAGGAATGTGGATTAGGTTGAGGGTG NGG 10 8 17 8 24 0 100 5 10
    240605115738_yv9k TGCATTTTCCTGACTGATGATTGATGTGGGAG(+CAGTGT)GATCCATCCCAATGTGGGCAACTATAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605115736_so6s CCTGCTTTCTTATAGTTGCCCACATTGGGATG(+CAGTGT)GATCCTCCCACATCAATCATCAGTCAGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605115735_2ju5 CAAGCAAACCATTCCACCTCAGAGCATATTTG(+CAGTGT)GATCCAATCTCTTTATTGTCAGGTTCCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605115734_uxa1 AGGGTGGGAGGAACCTGACAATAAAGAGATTG(+CAGTGT)GATCCAAATATGCTCTGAGGTGGAATGGTTT NGG 10 8 17 8 24 0 100 5 10
    240605115732_wm3p GCATGTACATCTGTGTACCATGCGTGTGTGTG(+CAGTGT)GTACCAGCAGAAGTCAGCAGAGGGCGCTGAG NGG 10 8 17 8 24 0 100 5 10
    240605115731_swi9 AGGAACTCAGCGCCCTCTGCTGACTTCTGCTG(+CAGTGT)GTACCACACACACGCATGGTACACAGATGTA NGG 10 8 17 8 24 0 100 5 10
    240605115730_a794 CACAGCACGTGTCAAGCTTGTATGAGACGCAG(+CAGTGT)GATCCTATCACCAGCACTGAGAATAAAACTT NGG 10 8 17 8 24 0 100 5 10
    240605115729_t0ws ACTTGAAGTTTTATTCTCAGTGCTGGTGATAG(+CAGTGT)GATCCTGCGTCTCATACAAGCTTGACACGTG NGG 10 8 17 8 24 0 100 5 10
    240605115728_j4t2 GACACATCCTGGTGTGTCCACATGTGTGCATG(+CAGTGT)GATCCATGTGTGCGCATTGGGGTGGGGGTGG NGG 10 8 17 8 24 0 100 5 10