Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605115651_snhx ACCCAGTTTGGAACTTTAATCTGTAACCCATG(+CAGTGT)GATCCACAGATTTTCTTGGGAGCTTGAATAG NGG 10 8 17 8 24 0 100 5 10
    240605115650_shox GACATTTTTACCATCTGCTCTCTTCAGTATCG(+CAGTGT)GATCCGTATTGAGAAAAGAGAAGCACCAGAC NGG 10 8 17 8 24 0 100 5 10
    240605115648_0rd8 GCAGCGTCTGGTGCTTCTCTTTTCTCAATACG(+CAGTGT)GATCCGATACTGAAGAGAGCAGATGGTAAAA NGG 10 8 17 8 24 0 100 5 10
    240605115646_v3mi GGGTCTGAGTTTGCTTTCCGTTGCTGTGGTGG(+CAGTGT)GTACCACGACTGGAGCAACATGGGGGAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605115645_vhlu TAAAGCCTTTCCCCCATGTTGCTCCAGTCGTG(+CAGTGT)GTACCCACCACAGCAACGGAAAGCAAACTCA NGG 10 8 17 8 24 0 100 5 10
    240605115643_fw5h CTGTTGTGTCTAGCTTGTAGGTGTGCTCTGGG(+CAGTGT)GATCCCAAGTCAGGTCATCAGACTTGCACTG NGG 10 8 17 8 24 0 100 5 10
    240605115641_y6ge GCTTGCAGTGCAAGTCTGATGACCTGACTTGG(+CAGTGT)GATCCCCAGAGCACACCTACAAGCTAGACAC NGG 10 8 17 8 24 0 100 5 10
    240605115639_jmoo CTGGAGTTATAGATGGTTGTGAGTTACCTGTG(+CAGTGT)GATCCTGAGAACTGAACCCAAGACTACAGGA NGG 10 8 17 8 24 0 100 5 10
    240605115638_p2ts GCTATTCCTGTAGTCTTGGGTTCAGTTCTCAG(+CAGTGT)GATCCACAGGTAACTCACAACCATCTATAAC NGG 10 8 17 8 24 0 100 5 10
    240605115637_ijl4 TTATTCTGTAGTTGGAGCCCAAACTCATTAAG(+CAGTGT)GTACCCTTTATGAGGCCAAGAGAGCCAGAGG NGG 10 8 17 8 24 0 100 5 10