Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605115619_b6ox CTAGGACTCCAGCCAAGGTCCCTTGGGAGGGG(+CAGTGT)GATCCCACCAGAATACCTTCACCTCTGATAC NGG 10 8 17 8 24 0 100 5 10
    240605115617_uyn4 GCTATGTGATATTCCACAGGTGTGTGGTCCGG(+CAGTGT)GATCCCTCAGGTAGAGAAAAGGAAACCTTGC NGG 10 8 17 8 24 0 100 5 10
    240605115615_bzpr CGTCAGCAAGGTTTCCTTTTCTCTACCTGAGG(+CAGTGT)GATCCCGGACCACACACCTGTGGAATATCAC NGG 10 8 17 8 24 0 100 5 10
    240605115613_dd2d GGGGCTCACTGCTCCACTAGGCTGGCTGCAGG(+CAGTGT)GATCCTACTGCCTCTACCTTCCCAGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605115612_n66k TAATTCCAGCACTGGGAAGGTAGAGGCAGTAG(+CAGTGT)GATCCCTGCAGCCAGCCTAGTGGAGCAGTGA NGG 10 8 17 8 24 0 100 5 10
    240605115611_g5c8 AGGACGCTAGAGGGCGCTCTAGGACAGCGTCG(+CAGTGT)GATCCTGTCTCTGGCAACCCCTTGCAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240605115609_d2gs CCCGTGGGCTTGCAAGGGGTTGCCAGAGACAG(+CAGTGT)GATCCGACGCTGTCCTAGAGCGCCCTCTAGC NGG 10 8 17 8 24 0 100 5 10
    240605115608_h0cr GGCATCTGACTCAAGGAATAGTCTGGAATAAG(+CAGTGT)GATCCGGGTCTGTTTACCCTGGTAGCCTCCG NGG 10 8 17 8 24 0 100 5 10
    240605115606_7018 TCAGCCGGAGGCTACCAGGGTAAACAGACCCG(+CAGTGT)GATCCTTATTCCAGACTATTCCTTGAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240605115603_4b20 AAGCCATCCTAGGAAGACCAAGTGATCCCATG(+CAGTGT)GATCCTGTACACCTCTCCTGGAGGAGCCCAG NGG 10 8 17 8 24 0 100 5 10