Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605115544_ove4 TGTAGGCTCACTTGGCCACCTCAGGACACCAG(+CAGTGT)GTACCCCCATCGGTGAAGGTAAAAGCCAGAA NGG 10 8 17 8 24 0 100 5 10
    240605115543_vwwe CAGGTGGATGTTTGGGTTCTGTTGGTAACTGG(+CAGTGT)GTACCTGCAAAAGAGCTTAGGACACTTTGCT NGG 10 8 17 8 24 0 100 5 10
    240605115541_eh6i TAATGAGCAAAGTGTCCTAAGCTCTTTTGCAG(+CAGTGT)GTACCCAGTTACCAACAGAACCCAAACATCC NGG 10 8 17 8 24 0 100 5 10
    240605115540_onn2 TCCAGCATGAGCAGGCTCCCTAGCTGTTGAAG(+CAGTGT)GATCCCATGGGTCAGGCACTGACCACCTTGA NGG 10 8 17 8 24 0 100 5 10
    240605115538_3ail CACCTTCAAGGTGGTCAGTGCCTGACCCATGG(+CAGTGT)GATCCTTCAACAGCTAGGGAGCCTGCTCATG NGG 10 8 17 8 24 0 100 5 10
    240605115536_dywu CTATGATCATATAATAACAACAATGTAGAAGG(+CAGTGT)GTACCACACCCTTGCTTGCCATGGCCTCACC NGG 10 8 17 8 24 0 100 5 10
    240605115535_gsst GAGATGGTGAGGCCATGGCAAGCAAGGGTGTG(+CAGTGT)GTACCCTTCTACATTGTTGTTATTATATGAT NGG 10 8 17 8 24 0 100 5 10
    240605115533_arx9 GTTCCACTTAGTCAGGAAGTCTCCAGCCTATG(+CAGTGT)GATCCTGTGCCACCCCCTGCCCCCAACGCCC NGG 10 8 17 8 24 0 100 5 10
    240605115531_775a TGTTTGGGCGTTGGGGGCAGGGGGTGGCACAG(+CAGTGT)GATCCATAGGCTGGAGACTTCCTGACTAAGT NGG 10 8 17 8 24 0 100 5 10
    240605115530_long TGAGATGAGTCCACCTCATCTGTAGCCTAGAG(+CAGTGT)GTACCTCAGTGAGTTGGGAAAGTCAGAGGCC NGG 10 8 17 8 24 0 100 5 10