Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605115458_jz3g TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+CAGTGT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605115455_rfr8 GGATGGACCATGTAGAGACTGCCTTATCCAGG(+CAGTGT)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605115453_bkyp GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+CAGTGT)GATCCCTGGATAAGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605115452_194f TTTGGTTTTCCGATGATGACCTGCATGTCATG(+CAGTGT)GTACCTTGTTTCCAGAATTATTGAGATCTAT NGG 10 8 17 8 24 0 100 5 10
    240605115451_kq5g ACTCCATAGATCTCAATAATTCTGGAAACAAG(+CAGTGT)GTACCATGACATGCAGGTCATCATCGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605115450_fcec ACTCCATAGATCTCAATAATTCTGGAAACAAG(+CAGTGT)GTACCATGACATGCAGGTCATCATCGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605115448_cpk1 TTTGGTTTTCCGATGATGACCTGCATGTCATG(+CAGTGT)GTACCTTGTTTCCAGAATTATTGAGATCTAT NGG 10 8 17 8 24 0 100 5 10
    240605115447_b4dh AACAAAAAACAAAAAACACTGGGATAAAGGAG(+CAGTGT)GTACCCAAGAATCAGTGGAGTTTACCCTAGC NGG 10 8 17 8 24 0 100 5 10
    240605115445_1pil TCACAGCTAGGGTAAACTCCACTGATTCTTGG(+CAGTGT)GTACCTCCTTTATCCCAGTGTTTTTTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605115444_n9qd CAGGAATAGAAATCCTGACCAAGACATTGTTG(+CAGTGT)GTACCAGCATAGTGGGGCATTCCTGTGACAA NGG 10 8 17 8 24 0 100 5 10