Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605115529_3jif TAAGTGGCCTCTGACTTTCCCAACTCACTGAG(+CAGTGT)GTACCTCTAGGCTACAGATGAGGTGGACTCA NGG 10 8 17 8 24 0 100 5 10
    240605115527_9ddm TCGCTGGGGCTGCCCCATAGGACACTGGGGTG(+CAGTGT)GTACCATGGAGAGACAGGGGATAAAACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605115525_nypt TTTGGATCAGTTTTATCCCCTGTCTCTCCATG(+CAGTGT)GTACCACCCCAGTGTCCTATGGGGCAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240605115524_pgo1 GCTCAAAATGTCTCTGGCTGTATTGATCTGAG(+CAGTGT)GATCCTCCCCTCTTTTGAACCATTCCTTCCA NGG 10 8 17 8 24 0 100 5 10
    240605115522_3mws GGTGATGGAAGGAATGGTTCAAAAGAGGGGAG(+CAGTGT)GATCCTCAGATCAATACAGCCAGAGACATTT NGG 10 8 17 8 24 0 100 5 10
    240605115521_5utv CAAGTCTGTGCCTCAGCACAGCTAGAAGTCAG(+CAGTGT)GATCCAAGGAGGCCTAAAATCGGCAATGACA NGG 10 8 17 8 24 0 100 5 10
    240605115519_yoa5 GAGTCTGTCATTGCCGATTTTAGGCCTCCTTG(+CAGTGT)GATCCTGACTTCTAGCTGTGCTGAGGCACAG NGG 10 8 17 8 24 0 100 5 10
    240605115517_9nzm CTCCCCATGGATTCATGTGCACCCATCTGGAG(+CAGTGT)GTACCAGATTCCTCCATGGCAGATCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    240605115516_emmz TGTTCCCAACAGATCTGCCATGGAGGAATCTG(+CAGTGT)GTACCTCCAGATGGGTGCACATGAATCCATG NGG 10 8 17 8 24 0 100 5 10
    240605115514_zw1r CCCCATGCTACTGATAACACAGTGATCACCAG(+CAGTGT)GATCCAAACTGGCTTCAGGTTTCTAACAAAA NGG 10 8 17 8 24 0 100 5 10