Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605115602_ppaj TGTACCTGGGCTCCTCCAGGAGAGGTGTACAG(+CAGTGT)GATCCATGGGATCACTTGGTCTTCCTAGGAT NGG 10 8 17 8 24 0 100 5 10
    240605115601_craj TGCTGAGACACAGAATGAGTTTTATGGCAAAG(+CAGTGT)GATCCTTAATCCCCAGAGACGCTTTGGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605115600_tdvl CACCACAAGCCAAAGCGTCTCTGGGGATTAAG(+CAGTGT)GATCCTTTGCCATAAAACTCATTCTGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605115557_329i TTTCTCTGGAGTCCTTCTTGGAGAAAACCAGG(+CAGTGT)GATCCAGGTTCATCGCCGAGGCCCTCGGAGC NGG 10 8 17 8 24 0 100 5 10
    240605115556_z26o GCCCCGCTCCGAGGGCCTCGGCGATGAACCTG(+CAGTGT)GATCCCTGGTTTTCTCCAAGAAGGACTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605115554_1dmw CATTGTGCCTCTGAGACATCCTTAGGGCTGGG(+CAGTGT)GTACCCAGCTCTCGAAGACACCATGCCATGA NGG 10 8 17 8 24 0 100 5 10
    240605115552_hfmn AGAAGTCATGGCATGGTGTCTTCGAGAGCTGG(+CAGTGT)GTACCCCAGCCCTAAGGATGTCTCAGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605115550_bvl2 ACTAACATCTCCCTCTCCCTCTCTTGCTATGG(+CAGTGT)GATCCCAATGGTACTGCAGGCAACACAGTAC NGG 10 8 17 8 24 0 100 5 10
    240605115549_43xe GACTGGTACTGTGTTGCCTGCAGTACCATTGG(+CAGTGT)GATCCCATAGCAAGAGAGGGAGAGGGAGATG NGG 10 8 17 8 24 0 100 5 10
    240605115546_bk6m GGCTCTTCTGGCTTTTACCTTCACCGATGGGG(+CAGTGT)GTACCTGGTGTCCTGAGGTGGCCAAGTGAGC NGG 10 8 17 8 24 0 100 5 10