Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605115428_hzyh ATTCAGGGTCAGCTTCAACTCCAGTTGTTTGG(+CAGTGT)GTACCTATATAAAGACCAAGTTGCACATCTG NGG 10 8 17 8 24 0 100 5 10
    240605115427_iqlc TGTAGCAGATGTGCAACTTGGTCTTTATATAG(+CAGTGT)GTACCCAAACAACTGGAGTTGAAGCTGACCC NGG 10 8 17 8 24 0 100 5 10
    240605115425_phuj TCACAGCTAGGGTAAACTCCACTGATTCTTGG(+CAGTGT)GTACCTCCTTTATCCCAGTGGTTTTTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240605115424_u6cc AAACAGAAAAAAAAACCACTGGGATAAAGGAG(+CAGTGT)GTACCCAAGAATCAGTGGAGTTTACCCTAGC NGG 10 8 17 8 24 0 100 5 10
    240605115422_lhhg ATCGACAGATGAACCAAGAGAATAGGAAAGAG(+CAGTGT)GATCCCAAAGCCGAACAAGAAAATCACGATC NGG 10 8 17 8 24 0 100 5 10
    240605115421_omce TAAGGGATCGTGATTTTCTTGTTCGGCTTTGG(+CAGTGT)GATCCTCTTTCCTATTCTCTTGGTTCATCTG NGG 10 8 17 8 24 0 100 5 10
    240605115419_og92 CAGAAAGGGGTCCCCTTTAGTGAAATGCTGGG(+CAGTGT)GATCCTTCTACAGCTCATGCTGGTTCACTTG NGG 10 8 17 8 24 0 100 5 10
    240605115418_39k1 CTTCCCAAGTGAACCAGCATGAGCTGTAGAAG(+CAGTGT)GATCCCCAGCATTTCACTAAAGGGGACCCCT NGG 10 8 17 8 24 0 100 5 10
    240605115416_w120 GGTGTCATCGTTTTTAGGCTGATTATGGGATG(+CAGTGT)GATCCCTGGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605115414_7fpl GGATGGACCATCTAGAGACTGCCATATCCAGG(+CAGTGT)GATCCATCCCATAATCAGCCTAAAAACGATG NGG 10 8 17 8 24 0 100 5 10