Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605115325_3pf7 TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+CAGTGT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605115323_nog0 TCCTCAGAAAATTGGACATAGTACTACCGGAG(+CAGTGT)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240605115321_pn11 CTTGTACTTTTCTTTATTCTCTACAAGACTGG(+CAGTGT)GATCCATCTTGGAAACATTAAGAATATCTAC NGG 10 8 17 8 24 0 100 5 10
    240605115320_1add ATCCAGTAGATATTCTTAATGTTTCCAAGATG(+CAGTGT)GATCCCAGTCTTGTAGAGAATAAAGAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605115319_qobo AATGTCTGCGTTTGGTGGCTGATTATGGGATG(+CAGTGT)GATCCCCGGATGGGGCAGTCTCTGGATAGTT NGG 10 8 17 8 24 0 100 5 10
    240605115316_tcnh ACTGTAACTATCCAGAGACTGCCCCATCCGGG(+CAGTGT)GATCCATCCCATAATCAGCCACCAAACGCAG NGG 10 8 17 8 24 0 100 5 10
    240605115314_69ip AAGCATGGACCAACTTAAAGGGTTTGCACTGG(+CAGTGT)GATCCTCTGTGGAGCTGAGTTCTACCACCTT NGG 10 8 17 8 24 0 100 5 10
    240605115313_n7it GTCAGAAGGTGGTAGAACTCAGCTCCACAGAG(+CAGTGT)GATCCCAGTGCAAACCCTTTAAGTTGGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605115310_6xp1 GCAGACAGTGTATCTGAAAAGTCTCTTCCCAG(+CAGTGT)GATCCAGAATGGGATGAACCTGTCTTGTGCT NGG 10 8 17 8 24 0 100 5 10
    240605115309_5kbn GAGGGAGCACAAGACAGGTTCATCCCATTCTG(+CAGTGT)GATCCTGGGAAGAGACTTTTCAGATACACTG NGG 10 8 17 8 24 0 100 5 10