Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605114903_qz4u ACAAGAAACCCCTGTCTAACGCAGGGCCTCAG(+CAGTGT)GATCCGAGACGGCTTTGGAAGTGAACTGCAA NGG 10 8 17 8 24 0 100 5 10
    240605114902_zig9 TGGCATTGCAGTTCACTTCCAAAGCCGTCTCG(+CAGTGT)GATCCTGAGGCCCTGCGTTAGACAGGGGTTT NGG 10 8 17 8 24 0 100 5 10
    240605114900_0qeh GGATGGACCATCTAGTGATTGCCATATGCAGG(+CAGTGT)GATCCATCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605114858_ijw8 GGTGTCAGCATTTGGAAGCTGATTATGGGATG(+CAGTGT)GATCCCTGCATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605114856_gy6u TAGGCTCAAGGTGATGCTGACCACATCTCCTG(+CAGTGT)GTACCTTCAACTAGAATTTCTAGACCAACAA NGG 10 8 17 8 24 0 100 5 10
    240605114855_ay28 TACTGTTGTTGGTCTAGAAATTCTAGTTGAAG(+CAGTGT)GTACCAGGAGATGTGGTCAGCATCACCTTGA NGG 10 8 17 8 24 0 100 5 10
    240605114854_ujgk AACTTGTTGGGAGTCAGTTCTTTCACCATGTG(+CAGTGT)GATCCCAGGGCTCAAATTCAGGTCATTTGGG NGG 10 8 17 8 24 0 100 5 10
    240605114851_s69u CTAAGCCCAAATGACCTGAATTTGAGCCCTGG(+CAGTGT)GATCCACATGGTGAAAGAACTGACTCCCAAC NGG 10 8 17 8 24 0 100 5 10
    240605114849_7qej GTGGGAATCAGTGATCCCCTTCTGCTAATGTG(+CAGTGT)GATCCCAGGGACTGAACTCAGGCTGGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605114846_mlu4 TTCAAGCCTTCCAGCCTGAGTTCAGTCCCTGG(+CAGTGT)GATCCACATTAGCAGAAGGGGATCACTGATT NGG 10 8 17 8 24 0 100 5 10