Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605114845_1bqi GAGAACACAAGTCTCTGTAGGTGTAGGCTTTG(+CAGTGT)GTACCTCCCCGGGGCAGCACTGTGCAGGGTG NGG 10 8 17 8 24 0 100 5 10
    240605114843_8y7v CCACACACCCTGCACAGTGCTGCCCCGGGGAG(+CAGTGT)GTACCAAAGCCTACACCTACAGAGACTTGTG NGG 10 8 17 8 24 0 100 5 10
    240605114841_35wj CTGTTTCCCTAAACTCCATCCTTTCTTCCCAG(+CAGTGT)GATCCTGCCCTTCATAGAGGGAACAGGGTAG NGG 10 8 17 8 24 0 100 5 10
    240605114839_b3sh TGGAGCTACCCTGTTCCCTCTATGAAGGGCAG(+CAGTGT)GATCCTGGGAAGAAAGGATGGAGTTTAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605114838_y7md CTGGGGCTTCTCTCCACTTTCTGGCAAGGAAG(+CAGTGT)GTACCAGGGTCACAGACTCCCACACAGTCTC NGG 10 8 17 8 24 0 100 5 10
    240605114835_w66l TACTTGAGACTGTGTGGGAGTCTGTGACCCTG(+CAGTGT)GTACCTTCCTTGCCAGAAAGTGGAGAGAAGC NGG 10 8 17 8 24 0 100 5 10
    240605114834_u2ow CCTGTCAGTGAGGGGGGTTGACTCCTGTGTGG(+CAGTGT)GTACCATTGGGGAAGGATGGATTTATCACAC NGG 10 8 17 8 24 0 100 5 10
    240605114832_kkiz GGAGAGTGTGATAAATCCATCCTTCCCCAATG(+CAGTGT)GTACCCACACAGGAGTCAACCCCCCTCACTG NGG 10 8 17 8 24 0 100 5 10
    240605114830_w8w3 AGACCAAGAACAGCAGAAGCTCACAGAGTGAG(+CAGTGT)GATCCAGGGAAGCCTTGGCAGGCTGTGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605114828_69sc CCGCTTGCCCACAGCCTGCCAAGGCTTCCCTG(+CAGTGT)GATCCTCACTCTGTGAGCTTCTGCTGTTCTT NGG 10 8 17 8 24 0 100 5 10