Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605114622_7gd5 CAAGCATGAAGACATAATTATGAAAACACAAG(+CAGTGT)GTACCACCAAGAATCCAACAACACTGAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605114620_x9eb TGTTAATTTTCAGTGTTGTTGGATTCTTGGTG(+CAGTGT)GTACCTTGTGTTTTCATAATTATGTCTTCAT NGG 10 8 17 8 24 0 100 5 10
    240605114619_e0np AAATTACATAAACTACTGGAAAACCAGTGAAG(+CAGTGT)GTACCAGGTCAATAGATTAAAAGACTATACA NGG 10 8 17 8 24 0 100 5 10
    240605114617_8bns CGTATTGTATAGTCTTTTAATCTATTGACCTG(+CAGTGT)GTACCTTCACTGGTTTTCCAGTAGTTTATGT NGG 10 8 17 8 24 0 100 5 10
    240605114615_useo CCTTAGGCAGTGCCTGCTACTCCAAAGGGGAG(+CAGTGT)GTACCGTTAGTGTGCAGGAATGAGACACTTG NGG 10 8 17 8 24 0 100 5 10
    240605114614_mfxs CCTTCCAAGTGTCTCATTCCTGCACACTAACG(+CAGTGT)GTACCTCCCCTTTGGAGTAGCAGGCACTGCC NGG 10 8 17 8 24 0 100 5 10
    240605114612_eesu GTGCAGGAATGAGACACTTGGAAGGCAGATGG(+CAGTGT)GATCCAAGAGAAAGGCATACTTGGTAAACTA NGG 10 8 17 8 24 0 100 5 10
    240605114611_rrva CAATATAGTTTACCAAGTATGCCTTTCTCTTG(+CAGTGT)GATCCCATCTGCCTTCCAAGTGTCTCATTCC NGG 10 8 17 8 24 0 100 5 10
    240605114610_obd0 CATCTACTTTGCCTTTTTGTGCAGGTTATATG(+CAGTGT)GATCCTGCACCGCTCATTACAAAGGTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240605114608_o3vz AAACGTGCAGACCTTTGTAATGAGCGGTGCAG(+CAGTGT)GATCCATATAACCTGCACAAAAAGGCAAAGT NGG 10 8 17 8 24 0 100 5 10