Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605114552_yu0q TAAAGTCACTTATAGATACTCAGAAGGAAAAG(+CAGTGT)GTACCCCAATATTCCAATAGTATCGGTACAT NGG 10 8 17 8 24 0 100 5 10
    240605114550_s4h8 TCATAATGTACCGATACTATTGGAATATTGGG(+CAGTGT)GTACCTTTTCCTTCTGAGTATCTATAAGTGA NGG 10 8 17 8 24 0 100 5 10
    240605114549_nugg ATCAAGTTTCTTTTCAGCAGGAAAGCCAAGAG(+CAGTGT)GTACCTTTTGTCCACATCAAATGTCCCTTGC NGG 10 8 17 8 24 0 100 5 10
    240605114548_5xre ATTCTGCAAGGGACATTTGATGTGGACAAAAG(+CAGTGT)GTACCTCTTGGCTTTCCTGCTGAAAAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240605114547_0sqn ATGACTGCTTTGACTGCTTATAAAAGTGACAG(+CAGTGT)GATCCTCAGTAAGCAGCAACTCGAGAACTTG NGG 10 8 17 8 24 0 100 5 10
    240605114545_a2un GTCCCCAAGTTCTCGAGTTGCTGCTTACTGAG(+CAGTGT)GATCCTGTCACTTTTATAAGCAGTCAAAGCA NGG 10 8 17 8 24 0 100 5 10
    240605114544_dnwq GATAACTTCACAACATTGGAGTGGTCAGAAAG(+CAGTGT)GTACCTGTTAAATGTTACTAACGAAACAAAT NGG 10 8 17 8 24 0 100 5 10
    240605114543_ut0s AGAGCATTTGTTTCGTTAGTAACATTTAACAG(+CAGTGT)GTACCTTTCTGACCACTCCAATGTTGTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605114542_i9sv TTCCTCTATAAATTTTAAGTCTTGTTGCAGAG(+CAGTGT)GTACCCAGGAAGTCCTGCTGTCCTCTAATGG NGG 10 8 17 8 24 0 100 5 10
    240605114539_x4u3 ACAACCCATTAGAGGACAGCAGGACTTCCTGG(+CAGTGT)GTACCTCTGCAACAAGACTTAAAATTTATAG NGG 10 8 17 8 24 0 100 5 10