Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113725_pbt6 CACTTGTCTTACTTGAGTCCTCTCCCCAAGTG(+CAGTGT)GTACCTGGAAGCCCCCACAGCCAAGACTAGA NGG 10 8 17 8 24 0 100 5 10
    240605113724_qy3v GGGTTGGGAACAAGGGGGTGGGGTCATCAGAG(+CAGTGT)GTACCTGTTACTTTAGGGACCCTCTCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605113722_391a CTCTCAGACAGAGAGGGTCCCTAAAGTAACAG(+CAGTGT)GTACCTCTGATGACCCCACCCCCTTGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605113721_4ytc CAGACTGGAGTTTTCTGCCTGTTTGTCTCTCG(+CAGTGT)GATCCTCAGAATAGCCGTGTGATGTGAGGTA NGG 10 8 17 8 24 0 100 5 10
    240605113720_g9jf CTGCCTACCTCACATCACACGGCTATTCTGAG(+CAGTGT)GATCCGAGAGACAAACAGGCAGAAAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240605113718_c5gz CTCATGACTTTATATATTCAAAGCTTTTAATG(+CAGTGT)GTACCTGTTACACAGCAAGTACTCTGTGAAT NGG 10 8 17 8 24 0 100 5 10
    240605113717_7jxh TCAGCATTCACAGAGTACTTGCTGTGTAACAG(+CAGTGT)GTACCATTAAAAGCTTTGAATATATAAAGTC NGG 10 8 17 8 24 0 100 5 10
    240605113715_rcem TAACCAATTGCTTAGGGGCAGGAAGATGTGGG(+CAGTGT)GATCCCACCTTGCCAGAGCTCTTACCATCTT NGG 10 8 17 8 24 0 100 5 10
    240605113713_5khr TGGCAAAGATGGTAAGAGCTCTGGCAAGGTGG(+CAGTGT)GATCCCCACATCTTCCTGCCCCTAAGCAATT NGG 10 8 17 8 24 0 100 5 10
    240605113712_hulp TAGGCTCACACACTAAGATTGACTGGCATATG(+CAGTGT)GATCCCTTTCTTAGATGGTGGTGTATAGCTT NGG 10 8 17 8 24 0 100 5 10