Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113622_vmn0 GGATGTGGGGCCCAGAGGCAAGGGGTTGATGG(+CAGTGT)GTACCCCATAGGGTGTGCTCACATCTGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605113621_6vbj TGTGGCTCACAGCAGCACTGAGTGGCCTTTGG(+CAGTGT)GTACCATGTGGCAGGCACACTGTCAGATGAG NGG 10 8 17 8 24 0 100 5 10
    240605113619_n7yo TTGTGCTCATCTGACAGTGTGCCTGCCACATG(+CAGTGT)GTACCCAAAGGCCACTCAGTGCTGCTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605113618_ovpb TCCCTGCAAGGCCAGCCCTGGGACAGTGCCTG(+CAGTGT)GTACCCGAGAAACAGTTATACGGGTGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605113616_jfsl CAGCGCAGGACACCCGTATAACTGTTTCTCGG(+CAGTGT)GTACCAGGCACTGTCCCAGGGCTGGCCTTGC NGG 10 8 17 8 24 0 100 5 10
    240605113613_ul3j AGATGGAGAGGACCTCAAGAGTCTGACCCTTG(+CAGTGT)GTACCATCCGATACTTAACTCCCTCGGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605113612_p4t4 GAAGAAGGACCGAGGGAGTTAAGTATCGGATG(+CAGTGT)GTACCAAGGGTCAGACTCTTGAGGTCCTCTC NGG 10 8 17 8 24 0 100 5 10
    240605113610_76c9 TTCTTCCTCTTCCTCTTCCTCCTTGAATCTGG(+CAGTGT)GTACCCTAACCCTGAGAAGAAAACCCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240605113608_miev AGAAAACTGAGGGTTTTCTTCTCAGGGTTAGG(+CAGTGT)GTACCCAGATTCAAGGAGGAAGAGGAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605113606_cow2 GCACAGCACCTACCAGCCCTGGCCCTTCCTTG(+CAGTGT)GTACCATCTCCACCACTGCTGGACTCCCACC NGG 10 8 17 8 24 0 100 5 10