Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113511_laev TGATTTGTGTCCCGCATCCCCTGGGGAAGCTG(+CAGTGT)GTACCATGGGCTTACTATGCTGTTTGGGACA NGG 10 8 17 8 24 0 100 5 10
    240605113510_nvsl GACGACCACTGGCTCCTAAAAGCACAGTCAGG(+CAGTGT)GATCCCTCTCAATGAGTGGGACCTAGGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605113508_kc2w TGATGCCACCCTAGGTCCCACTCATTGAGAGG(+CAGTGT)GATCCCTGACTGTGCTTTTAGGAGCCAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605113507_ktue AGGGCAAGGCGAACATGAAAGTTAACATGCAG(+CAGTGT)GTACCTGGCAGAGTGGGGACCCCAGTGGATG NGG 10 8 17 8 24 0 100 5 10
    240605113505_6cld CCCAACATCCACTGGGGTCCCCACTCTGCCAG(+CAGTGT)GTACCTGCATGTTAACTTTCATGTTCGCCTT NGG 10 8 17 8 24 0 100 5 10
    240605113503_mk3b TGGTGATAAGCTGTGAGGAGTGAATGGACAGG(+CAGTGT)GATCCAGAGGGACACAGCAGGCTCCACAAAG NGG 10 8 17 8 24 0 100 5 10
    240605113502_i15p TTCCTCTTTGTGGAGCCTGCTGTGTCCCTCTG(+CAGTGT)GATCCCTGTCCATTCACTCCTCACAGCTTAT NGG 10 8 17 8 24 0 100 5 10
    240605113500_umkc GGAGAAACTGGGTTAAGGCCAGAGAAGTGCCG(+CAGTGT)GATCCTGCCCCAGATTTTGACTTTAGAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605113458_g24l CATGGGAGCTCTAAAGTCAAAATCTGGGGCAG(+CAGTGT)GATCCGGCACTTCTCTGGCCTTAACCCAGTT NGG 10 8 17 8 24 0 100 5 10
    240605113457_8tnf GCCCGAGGTTCTGGTGGCCCAGCAACTGCAGG(+CAGTGT)GATCCATAAATCCAGAGGCCAGGATTCAGGC NGG 10 8 17 8 24 0 100 5 10