Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605114407_65w3 TTAGTGACTTTAAAATACACAACCTTTCCAAG(+CAGTGT)GTACCAACGTCGGAAAAAACAAACAACAAAA NGG 10 8 17 8 24 0 100 5 10
    240605114406_hpfn GCCCTCGTCAGAACAGAGCCCCACCACTGCTG(+CAGTGT)GTACCCGCATGGATGATGTGTGTGAATTGCC NGG 10 8 17 8 24 0 100 5 10
    240605114404_kh4b TACTTGGCAATTCACACACATCATCCATGCGG(+CAGTGT)GTACCAGCAGTGGTGGGGCTCTGTTCTGACG NGG 10 8 17 8 24 0 100 5 10
    240605114402_q3jn AAAGTGTACATCAAGACCAACAACATCCTGTG(+CAGTGT)GTACCGCAGGATCCAGGAAAGCAGCCTGTGT NGG 10 8 17 8 24 0 100 5 10
    240605114400_zhd7 TGTCCACACAGGCTGCTTTCCTGGATCCTGCG(+CAGTGT)GTACCACAGGATGTTGTTGGTCTTGATGTAC NGG 10 8 17 8 24 0 100 5 10
    240605114358_a5s0 ATCAAGACCAACAACATCCTGTGGTACCGCAG(+CAGTGT)GATCCAGGAAAGCAGCCTGTGTGGACACACA NGG 10 8 17 8 24 0 100 5 10
    240605114357_djxc AATGCTGTGTGTCCACACAGGCTGCTTTCCTG(+CAGTGT)GATCCTGCGGTACCACAGGATGTTGTTGGTC NGG 10 8 17 8 24 0 100 5 10
    240605114355_pa2z TTGACTGAGTAAAGGAGAGGTGGTTTCTATGG(+CAGTGT)GATCCTTTCTGTCTGCTGGGGGACATGGGGC NGG 10 8 17 8 24 0 100 5 10
    240605114354_4aj1 CCAATGCCCCATGTCCCCCAGCAGACAGAAAG(+CAGTGT)GATCCCATAGAAACCACCTCTCCTTTACTCA NGG 10 8 17 8 24 0 100 5 10
    240605114352_t4ch AGACTGAGGCTTGTGGCCATTGTGCCAAATGG(+CAGTGT)GTACCTTGCTGAAACACAGGGTTACAGTGTG NGG 10 8 17 8 24 0 100 5 10