Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113407_zxqu TGTATGTAAAGTTCCAGGGATTTCAAAATCTG(+CAGTGT)GTACCTAATGTGGCAAATGCACGGCTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605113406_52jx TAATCCTCACAGCCGTGCATTTGCCACATTAG(+CAGTGT)GTACCAGATTTTGAAATCCCTGGAACTTTAC NGG 10 8 17 8 24 0 100 5 10
    240605113404_6zzi CATGTATTTATATGCACCACCAGAGTGCCTGG(+CAGTGT)GATCCTGAAATCAGAAGACAGCATTAGGTCC NGG 10 8 17 8 24 0 100 5 10
    240605113403_ku6d TTCTAGGACCTAATGCTGTCTTCTGATTTCAG(+CAGTGT)GATCCCAGGCACTCTGGTGGTGCATATAAAT NGG 10 8 17 8 24 0 100 5 10
    240605113401_q4cm GAAACAAAACCAAAACCCAACTTCTCAGTAGG(+CAGTGT)GTACCACTAGCTGGCCTGTGGACAACAGTCT NGG 10 8 17 8 24 0 100 5 10
    240605113400_h47a TGAGGAGACTGTTGTCCACAGGCCAGCTAGTG(+CAGTGT)GTACCCTACTGAGAAGTTGGGTTTTGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605113357_8xju CTATCTGGTCTGCCCCTGTGTAGCTCCCATGG(+CAGTGT)GATCCCTTAAAATGCATCCTTTTTAATTTTC NGG 10 8 17 8 24 0 100 5 10
    240605113355_zpp0 GCCCGGAAAATTAAAAAGGATGCATTTTAAGG(+CAGTGT)GATCCCATGGGAGCTACACAGGGGCAGACCA NGG 10 8 17 8 24 0 100 5 10
    240605113352_ih1d ATGCGTCCTTGCCCACCTCTCACCAAGCCCCG(+CAGTGT)GATCCTTGCGGACTTCCCCAGGTGCCCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605113351_4pcv GGGGACAGGGGGCACCTGGGGAAGTCCGCAAG(+CAGTGT)GATCCGGGGCTTGGTGAGAGGTGGGCAAGGA NGG 10 8 17 8 24 0 100 5 10