Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113339_5iva CTCCTCAGAGGGAGATGACAGGAGTGGAGATG(+CAGTGT)GTACCGATAGTCGTTGCTAGTCTGTCTACCG NGG 10 8 17 8 24 0 100 5 10
    240605113338_d282 ACGCACGGTAGACAGACTAGCAACGACTATCG(+CAGTGT)GTACCATCTCCACTCCTGTCATCTCCCTCTG NGG 10 8 17 8 24 0 100 5 10
    240605113336_641z TCGTGGACATGGCAAAACCAGGCAAGAGCCAG(+CAGTGT)GATCCACAGAGAAACCCTCTTGCTTGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605113334_f6ic CCCAGAGAAGCAAGCAAGAGGGTTTCTCTGTG(+CAGTGT)GATCCTGGCTCTTGCCTGGTTTTGCCATGTC NGG 10 8 17 8 24 0 100 5 10
    240605113333_tu2k GGGGGGTAGCCTGGACTGGCAGGTGGGCAGAG(+CAGTGT)GTACCTGTGTGGCCAGGGCCACTCTAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605113332_bbap ACTTACAGACTAGAGTGGCCCTGGCCACACAG(+CAGTGT)GTACCTCTGCCCACCTGCCAGTCCAGGCTAC NGG 10 8 17 8 24 0 100 5 10
    240605113330_1ypy AGGGAGAGGAGGAAGAGGGCAGCATCCAGAGG(+CAGTGT)GATCCCTATGGACCCCAGCACTGACTGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605113328_d6kx TGGGTGCTGCAGTCAGTGCTGGGGTCCATAGG(+CAGTGT)GATCCCTCTGGATGCTGCCCTCTTCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240605113327_9lzo CTTGATCCCAGGTGGATTCCCACCCTAACTGG(+CAGTGT)GTACCAGGACTCTGAACAGGCCAAGATGGGG NGG 10 8 17 8 24 0 100 5 10
    240605113325_bh27 GAGTGCCCCATCTTGGCCTGTTCAGAGTCCTG(+CAGTGT)GTACCCAGTTAGGGTGGGAATCCACCTGGGA NGG 10 8 17 8 24 0 100 5 10