Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113248_99vh TGGCCCTACAGAACACACCTGAGCCTCTTGGG(+CAGTGT)GTACCATCGCAGGGTAGCCCTCCCTAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240605113247_e6tf AACTAGGCCCTAGGGAGGGCTACCCTGCGATG(+CAGTGT)GTACCCCAAGAGGCTCAGGTGTGTTCTGTAG NGG 10 8 17 8 24 0 100 5 10
    240605113246_8lwy CCAAGAACCAACTAGCTGCCTCAGGTCTCGTG(+CAGTGT)GATCCCAGATGGACATGGCAGTAACTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240605113244_j5bj ACCGGCTCTGAGTTACTGCCATGTCCATCTGG(+CAGTGT)GATCCACGAGACCTGAGGCAGCTAGTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240605113242_2rbx TCTCACCTGAGAAATGGTGCAAACCAACCAGG(+CAGTGT)GATCCTTAGGCTACTGAGAAGGCTGTCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605113240_pvk8 AGGTAAGCAGACAGCCTTCTCAGTAGCCTAAG(+CAGTGT)GATCCCTGGTTGGTTTGCACCATTTCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240605113238_o420 GGGCCCTGTGACCTTCAACATCTGGCAGGGGG(+CAGTGT)GATCCTGGGACAGGGGGTGGAGAAGGCAGTT NGG 10 8 17 8 24 0 100 5 10
    240605113235_uixx GTGTCAACTGCCTTCTCCACCCCCTGTCCCAG(+CAGTGT)GATCCCCCCTGCCAGATGTTGAAGGTCACAG NGG 10 8 17 8 24 0 100 5 10
    240605113234_geed TCACTAAGAGGACTGAAGGATCTAGTTGAGAG(+CAGTGT)GATCCCTCATGAACGTCCACTACCAGCCAGA NGG 10 8 17 8 24 0 100 5 10
    240605113233_y8n0 TGAGCTCTGGCTGGTAGTGGACGTTCATGAGG(+CAGTGT)GATCCTCTCAACTAGATCCTTCAGTCCTCTT NGG 10 8 17 8 24 0 100 5 10