Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113144_lknn TTTGCTGGACCTGATCCCCAAGCACAGGAGTG(+CAGTGT)GTACCCTCCACAGAAGCTGGGGGAGAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605113143_n5nb TGCTGCAGACTCTCCCCCAGCTTCTGTGGAGG(+CAGTGT)GTACCACTCCTGTGCTTGGGGATCAGGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605113141_zx39 ACTATTAACTATAATAGAAGTCACTAGGAAGG(+CAGTGT)GTACCATGCTCTCAGAATCCCCACGTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240605113140_mpx2 CGTGGCTGGGACGTGGGGATTCTGAGAGCATG(+CAGTGT)GTACCCTTCCTAGTGACTTCTATTATAGTTA NGG 10 8 17 8 24 0 100 5 10
    240605113138_y0yc TGGTCCTTGGCACAGCCCCTCCCTCATGGCTG(+CAGTGT)GATCCTCAGATCATAATAGGGCCTTGGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605113137_shh7 AACCCTGCTCCAAGGCCCTATTATGATCTGAG(+CAGTGT)GATCCAGCCATGAGGGAGGGGCTGTGCCAAG NGG 10 8 17 8 24 0 100 5 10
    240605113136_e7zz TTCACCTTCCGGAGAAAACATTTCAAGCGGAG(+CAGTGT)GATCCGGTATCTCGGGATTCCGAAGTCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605113134_ym8i ACACCGGGAGACTTCGGAATCCCGAGATACCG(+CAGTGT)GATCCTCCGCTTGAAATGTTTTCTCCGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605113132_r3t9 ATTGCACTTATTTCTATGAACCCCATGGAGGG(+CAGTGT)GATCCCCAATGCTGCTGCCTCCAGGCGAGGC NGG 10 8 17 8 24 0 100 5 10
    240605113130_ydno GTCATGCCTCGCCTGGAGGCAGCAGCATTGGG(+CAGTGT)GATCCCCTCCATGGGGTTCATAGAAATAAGT NGG 10 8 17 8 24 0 100 5 10