Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605113159_gdnz TGCTAGTTGCTCTGAAAGATTGGGACCTCTTG(+CAGTGT)GATCCCCCCCCCCATGGACTCTGATTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605113156_s8n6 ATAGACAGACAATCAGAGTCCATGGGGGGGGG(+CAGTGT)GATCCAAGAGGTCCCAATCTTTCAGAGCAAC NGG 10 8 17 8 24 0 100 5 10
    240605113155_cwcl AACCCCCGCCAGCCCAGTAGGGTAGCCAGGAG(+CAGTGT)GATCCCTGAGTCCAACCCGTAAACACTAGAG NGG 10 8 17 8 24 0 100 5 10
    240605113153_y4rv TGTGGCTCTAGTGTTTACGGGTTGGACTCAGG(+CAGTGT)GATCCTCCTGGCTACCCTACTGGGCTGGCGG NGG 10 8 17 8 24 0 100 5 10
    240605113152_6l4x GCTATAGAGGGCAAACCTGTTTGTATTCATGG(+CAGTGT)GATCCAACAAAATACATGAAAGGATGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605113151_3ako CATATTGGCTCATCCTTTCATGTATTTTGTTG(+CAGTGT)GATCCCATGAATACAAACAGGTTTGCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605113149_9eqk TCACTGAGCTCTGTTCTCTGTCTGCAGCACAG(+CAGTGT)GTACCAAAGAGGGGTGCAATGGAGGGCTAGC NGG 10 8 17 8 24 0 100 5 10
    240605113148_2t8q TCAGAGCTAGCCCTCCATTGCACCCCTCTTTG(+CAGTGT)GTACCTGTGCTGCAGACAGAGAACAGAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605113147_e5em AGTCATAGCTTCTCAGCAGTTGTGAAAAGTGG(+CAGTGT)GTACCATAGCCAGCTCCACTAGCTTCAGGGC NGG 10 8 17 8 24 0 100 5 10
    240605113146_1939 CCAATGCCCTGAAGCTAGTGGAGCTGGCTATG(+CAGTGT)GTACCCACTTTTCACAACTGCTGAGAAGCTA NGG 10 8 17 8 24 0 100 5 10